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Optimization of a Suite of Microsatellite Markers for Nocomis leptocephalus (Bluehead Chub) and Genetic Characterization of Two Populations in South Carolina

机译:优化南卡罗来纳州的两种群体微卫星标志物套装肽标记

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摘要

Nocomis leptocephalus (Bluehead Chub) is a minnow native to the southeastern United States that constructs nests used by many freshwater fishes. No microsatellite markers have been published for Bluehead Chub, and information on genetic structure and diversity is sparse. We evaluated microsatellites from other leuciscid species for use with Bluehead Chub and created a panel of markers that has sufficient power for investigations of population structure and can differentiate between Bluehead Chub and Notropis lutipinnis (Yellowfin Shiner) eggs. We applied the panel to Bluehead Chub samples from 2 locations in South Carolina, finding these populations are genetically differentiated with high levels of genetic diversity. Our marker panel can improve our understanding of population dynamics of Bluehead Chub and allow for informed conservation recommendations.
机译:Nocomis Leptocephalus(Bluehead Chub)是一个原产于美国东南部的Min鱼,构建了许多淡水鱼使用的巢穴。 Bluehead Chub没有公布微卫星标记,并且有关遗传结构和多样性的信息稀疏。 我们评估了来自其他睫毛物种的微卫星,以与Bluehead Chub一起使用,并创建了一个标记面板,具有足够的力量来调查人口结构,可以区分蓝头糖果和Notropis Lutipinnis(黄鳍素闪存)卵。 我们将小组应用于来自南卡罗来纳州的2个地点的Bluehead Chub样本,发现这些人群与高水平的遗传多样性遗传分化。 我们的标记面板可以改善我们对蓝头糖果的人口动态的理解,并允许知情保护建议。

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