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首页> 外文期刊>Parasitology >Development of EST-derived microsatellite markers to investigate the population structure of sparganum — the causative agent of zoonotic sparganosis
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Development of EST-derived microsatellite markers to investigate the population structure of sparganum — the causative agent of zoonotic sparganosis

机译：EST衍生的微卫星标记的发展，研究SPARAMUM的群体结构 - 人畜共患病的致病因子

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摘要

The plerocercoid (sparganum) of Spirometra erinaceieuropaei is the main aetiological agent of human sparganosis. To improve the current knowledge on S. erinaceieuropaei evolution, we performed multi-locus microsatellite typing of sparganum isolates from China for the first time. All available expressed sequence tag (EST) sequences for the Spirometra were downloaded from the GenBank. The identification and localization of microsatellites in ESTs was accomplished by MISA. Based on the selected microsatellites, the genetic structure of 64 sparganum isolates collected from 11 geographical locations in southwest China were investigated through principal component analysis, STRUCTURE analysis and neighbour-joining clustering. A total of 522 non-redundant ESTs containing 915 simple sequence repeats were identified from 12 481 ESTs screened. Five primer pairs were finally selected. Using these loci, a total of 12 alleles were detected in 64 sparganum isolates. Little variability was observed within each of geographical population, especially among isolates derived from Kunming of Yunnan (YN-KM) province. Both STRUCTURE analysis and the clustering analysis supported that two genotypes existed among the sparganum isolates from southwest China. In conclusion, five microsatellite markers were successfully developed, and sparganum population was observed to harbour low genetic variation, further investigation with deeper sampling was needed to elucidate the population structure.
机译：螺螺肌肌腱素（Sparmum）是人类股票病症的主要动态剂。为了提高目前对erinaceieuropaei进化的了解，我们首次执行了来自中国的多轨道微卫星键入的Sparum隔离物。 Spirometra的所有可用的表达序列标签（EST）序列从Genbank下载。 Misa完成了EST中微卫星的鉴定和定位。基于所选微卫星，通过主成分分析，结构分析和邻近加入聚类，研究了从西南11个地理位置收集的64个Sparthum分离物的遗传结构。共有来自522个含有915个简单序列重复的非冗余EST，从筛选的12个481 ests鉴定出915个简单序列重复。最终选择五对。使用这些基因座，在64个Sparganum隔离物中检测了总共12个等位基因。在每个地理人群中观察到几乎没有变异性，特别是在云南昆明（YN-KM）省的孤立株中。结构分析和聚类分析都支持两种基因型存在于中国西南部的Spargnum孤立障碍。总之，成功开发了五种微卫星标志物，观察到素群患有低遗传变异，需要进行更深的采样进行进一步调查来阐明人口结构。

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