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首页> 外文期刊>Parasitology >Novel polymorphic microsatellite loci in Anisakis pegreffii and A. simplex (s. s.) (Nematoda: Anisakidae): implications for species recognition and population genetic analysis
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Novel polymorphic microsatellite loci in Anisakis pegreffii and A. simplex (s. s.) (Nematoda: Anisakidae): implications for species recognition and population genetic analysis

机译：anisakis pegreffiii和a的新型多晶型微卫星基因座。Simplex（s. s。）（Nematoda：Anisakidae）：对物种识别和人口遗传分析的影响

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摘要

The species of Anisakis constitute one of the most widespread groups of ascaridoid nematodes in the marine ecosystem. Three closely related taxa are recognised in the A. simplex (s. l.) complex, i.e. A. pegreffii, A. simplex (s. s.) and A. berlandi. They are distributed in populations of their intermediate/paratenic (fish and squids) and definitive (cetaceans) hosts. A panel of seven microsatellite loci (Anisl 05784, Anisl 08059, Anisl 00875, Anisl 07132, Anisl 00314, Anisl 10535 and Anisl 00185), were developed and validated on a total of N = 943 specimens of A. pegreffii and A. simplex (s. s.), collected in fish and cetacean hosts from allopatric areas within the range of distribution of these parasite species. In addition, the locus Anisl 7, previously detected in those Anisakis spp., was investigated. The parasites were first identified by sequence analysis of the EF1 α-1 nDNA. The panel of the microsatellites loci here developed have allowed to: (i) detect diagnostic microsatellite loci between the two species; (ii) identify specimens of the two species A. pegreffii, A. simplex (s. s.) in a multi-marker nuclear genotyping approach; (iii) discover two sex-linked loci in both Anisakis species and (iv) estimate levels of genetic differentiation at both the inter- and intra-specific level.
机译：anisakis的种类构成了海洋生态系统中最广泛的蛔虫线虫。三个密切相关的分类群在A. Simplex（s.）复合物中，即A. Pegrefyii，A. Simplex（s。）和A. Berlandi。它们分布在其中间/帕拉多奇（鱼和鱿鱼）和最终（鲸料）主持人的群体中。开发并验证了七种微卫星基因座（ANIS105784，ANIS105784，ANIS108059，ANIS107132，ANISL 10535和ANISL 00185），总共N = PEGREFFIII和A.Ximplx（ SS），在这些寄生虫物种的分布范围内的分类区域收集鱼和鲸类宿主。另外，先前在那些anisakis spp中检测到的基因座Anis17。首先通过EF1α-1 NDNA的序列分析鉴定寄生虫。这里的微卫星基因座的小组已经开发了：（i）检测两种物种之间的诊断微卫星基因座; （ii）在多标记核基因分型方法中识别两种物种A. Pegrefyii的标本; （iii）在anisakis种类和（iv）中发现两种性别连接的基因座，（iv）估计特定内和内部内部遗传分化的水平。

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