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首页> 外文期刊>Parasitology >Hammondia triffittae n. comb. of foxes ( Vulpes spp.): biological and molecular characteristics and differentiation from Hammondia heydorni of dogs.
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Hammondia triffittae n. comb. of foxes ( Vulpes spp.): biological and molecular characteristics and differentiation from Hammondia heydorni of dogs.

机译：Hammondia Triffittae n。 梳子。 狐狸（狐狸spp）：狗哈蒙迪亚Heydorni的生物学和分子特征和分化。

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摘要

Genomic DNAs from 3 oocyst isolates of Hammondia sp. from foxes (Vulpes vulpes and V. lagopus) and 1 oocyst isolate of Hammondia heydorni from a dog, were examined by PCR and sequence analysis of 6 loci in order to determine whether the isolates were conspecific. Consistent genetic differences were found between the fox and dog isolates, respectively, at the ITS-2 region, the lsu rRNA gene, the alpha tubulin gene and the HSP70 gene, but not at the ssu rRNA gene or ITS-1 locus. Infection experiments established that dogs were unsuitable as definitive hosts for Hammondia sp. of foxes; hence this species is regarded as separate from H. heydorni of wolf-like canids, but probably identical with Isospora triffittae (syn. Isospora triffitti) previously reported from foxes. This species has therefore been named Hammondia triffittae n. comb. Reindeer, moose, sheep, goats, foxes and rabbits may act as intermediate hosts for H. triffittae. Muscle tissues of inoculated intermediate hosts were infectious for foxes from 16 days post-infection. Oocysts of H. triffittae were subspherical, averaging 12.5 x 10.9 mum in size. The lsu rRNA gene and the alpha tubulin gene seem to be suitable genetic markers for differentiating between H. triffittae and H. heydorni.
机译：来自Hammondia SP的3个卵囊分离株的基因组DNA。通过PCR和1个Hymondia Heydorni的狐狸（狐狸狐狸狐狸和v.Lagopus）和1个卵囊孤立，PCR和6个基因座的序列分析检查，以确定分离株是否是面临的。在其-2区，LSU rRNA基因，α管蛋白基因和HSP70基因之间分别在狐狸和狗分离物之间发现一致的遗传差异，但不在SSU rRNA基因或其-1个基因座。感染实验确立了狗不适合作为Hammondia SP的过定宿主。狐狸;因此，该物种被认为是与狼一样的Heydorders分开，但可能与先前从狐狸报告的isospora triffittae（syn。isospora triffitti）相同。因此，该物种被命名为Hammondia Triffittae n。梳子。驯鹿，驼鹿，羊，山羊，狐狸和兔子可以充当H. Triffittae的中间宿主。从感染后16天开始，接种中间体宿主的肌肉组织对狐狸感染。 H. Triffittae的卵囊是亚球的，平均大小为12.5×10.9米。 LSU rRNA基因和α微管蛋白基因似乎是适合于区分H. Triffittae和H Heydorni之间的遗传标记。

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  • 来源
    《Parasitology》 |2011年第3期|共19页
  • 作者

    Gjerde B; Dahlgren SS;

  • 作者单位

    Department of Food Safety and Infection Biology Section of Microbiology Immunology and Parasitology Norwegian School of Veterinary Science 0033 Oslo Norway.;

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  • 正文语种 eng
  • 中图分类 寄生虫病;
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