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首页> 外文期刊>Parasitology >Hammondia triffittae n. comb. of foxes ( Vulpes spp.): biological and molecular characteristics and differentiation from Hammondia heydorni of dogs.
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Hammondia triffittae n. comb. of foxes ( Vulpes spp.): biological and molecular characteristics and differentiation from Hammondia heydorni of dogs.

机译：滨藜梳。狐狸（狐狸属）的生物学和分子特征以及与狗的Hammondia heydorni的区别。

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摘要

Genomic DNAs from 3 oocyst isolates of Hammondia sp. from foxes (Vulpes vulpes and V. lagopus) and 1 oocyst isolate of Hammondia heydorni from a dog, were examined by PCR and sequence analysis of 6 loci in order to determine whether the isolates were conspecific. Consistent genetic differences were found between the fox and dog isolates, respectively, at the ITS-2 region, the lsu rRNA gene, the alpha tubulin gene and the HSP70 gene, but not at the ssu rRNA gene or ITS-1 locus. Infection experiments established that dogs were unsuitable as definitive hosts for Hammondia sp. of foxes; hence this species is regarded as separate from H. heydorni of wolf-like canids, but probably identical with Isospora triffittae (syn. Isospora triffitti) previously reported from foxes. This species has therefore been named Hammondia triffittae n. comb. Reindeer, moose, sheep, goats, foxes and rabbits may act as intermediate hosts for H. triffittae. Muscle tissues of inoculated intermediate hosts were infectious for foxes from 16 days post-infection. Oocysts of H. triffittae were subspherical, averaging 12.5 x 10.9 mum in size. The lsu rRNA gene and the alpha tubulin gene seem to be suitable genetic markers for differentiating between H. triffittae and H. heydorni.
机译：来自Hammondia sp。3种卵囊分离物的基因组DNA。通过PCR和6个基因座的序列分析检查了来自狐狸（狐狸狐和V. lagopus）的狐狸和狐狸的1个卵囊分离物，并通过PCR和6个基因座的序列分析来确定分离物是否是同种的。在狐狸和狗的分离株之间，在ITS-2，lsu rRNA基因，α微管蛋白基因和HSP70基因上分别发现了一致的遗传差异，而在ssu rRNA基因或ITS-1基因座上则没有。感染实验确定，狗不适合作为Hammondia sp。的最终宿主。狐狸因此，该物种被认为与狼类犬的H. heydorni分离，但可能与先前从狐狸中报道的Isospora triffittae（syn。Isospora triffitti）相同。因此该物种被命名为Hammondia triffittae n。梳。驯鹿，驼鹿，绵羊，山羊，狐狸和兔子可能充当三叶草的中间宿主。从感染后16天开始，接种的中间宿主的肌肉组织被狐狸传染。 Triffittae的卵囊是亚球形的，平均大小为12.5 x 10.9毫米。 lsu rRNA基因和α微管蛋白基因似乎是区分三叶草嗜血杆菌和海德氏嗜血杆菌的合适遗传标记。

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