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首页> 外文期刊>Parasitology >Development of a versatile in vitro method for understanding the migration of Fasciola hepatica newly excysted juveniles
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Development of a versatile in vitro method for understanding the migration of Fasciola hepatica newly excysted juveniles

机译：一种了解术语对新源性青少年血糖肝的迁移的多功能体外方法

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Fasciola hepatica is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However, relatively few studies have concentrated on the mechanisms of invasion of the gut by newly excysted juvenile liver flukes (NEJ) and the host response triggered by this event. The aim of this work was to develop an in vitro model to study invasion by NEJ, while also reducing the requirement for challenge infections of experimental animals. Fasciola hepatica metacercariae were excysted in vitro and placed into compartments containing rat distal jejunal sheets. Variations in incubation medium, chamber size and incubation temperature were used to identify optimal conditions for NEJ migration across the gut. Histological examination showed increased migration until 120 min post-incubation. The use of RPMI, without gassing at 39 degrees C, as the incubation medium was found to be optimal, with 40.5% of NEJ migrating after 150 min. This study describes a readily-reproducible method for studying the migration of F. hepatica NEJ within the definitive host. It will be useful for identifying potential drug and vaccine targets.
机译：FasciolaHepatica是一种寄生虫，导致牲畜生产者的严重损失，以及动物园疾病。化疗对控制裂变病的局限性导致了对保护牛和绵羊免受感染的疫苗的显着兴趣。然而，相对较少的研究集中在新源性青少年肝氟普斯（NEJ）和由此事件引发的宿主反应的侵袭的侵袭机制。这项工作的目的是开发一个体外模型来研究内部的入侵，同时还减少了对实验动物挑战感染的要求。 FasciolaHepatica metacercariae在体外源于脱节，并置于含有大鼠远端Jejunal板的隔间。温育介质，腔室尺寸和孵育温度的变化用于识别整个肠道上网迁移的最佳条件。组织学检查显示迁移增加至120分钟后孵育后。使用RPMI，在39摄氏度下没有喷气，因为培养培养基是最佳的，但在150分钟后，40.5％的NEJ迁移。本研究描述了一种易于学习的可重复可重复的方法，用于研究最终宿主内F.Hepatica Nej的迁移。它对于鉴定潜在的药物和疫苗靶标，它将是有用的。

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