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首页> 外文期刊>Parasitology >Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China
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Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China

机译：核和线粒体DNA分析表明，在中国发生了FASCIOLA Hepatica和Fasciola Gigantica之间的杂交

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摘要

The well-known pathogens of fasciolosis, Fasciola hepatica (Fh) and Fasciola Gigantica (Fg), possess abundant mature sperms in their seminal vesicles, and thus, they reproduce bisexually. On the other hand, aspermic Fasciola flukes reported from Asian countries, which have no sperm in their seminal vesicles, probably reproduce parthenogenetically. The aim of this study was to reveal the origin of aspermic Fasciola flukes. The nuclear single copy markers, phosphoenolpyruvate carboxykinase and DNA polymerase delta, were employed for analysis of Fasciola species from China. The hybrid origin of aspermic Fasciola flukes was strongly suggested by the presence of the Fh/Fg type, which includes DNA fragments of both F. hepatica and F. gigantica. China can be regarded as the cradle of the interspecific hybridization because F. hepatica and F. gigantica were detected in the northern and southern parts of China, respectively, and hybrids flukes were distributed between the habitats of the two species. The Chinese origin was supported by the fact that a larger number of mitochondrial NADHdehydrogenase subunit 1 (nad1) haplotypes was detected in Chinese aspermic Fasciola populations than in aspermic populations from the neighbouring countries. Hereafter, 'aspermic' Fasciola flukes should be termed as 'hybrid' Fasciola flukes.
机译：诱导裂变病病原菌，Fasciola肝脏（FH）和Fasciola gigantica（FG）具有丰富的成熟精子，因此它们两性繁殖。另一方面，来自亚洲国家的Aspermic Fasciola Flukes，这些国家没有精子在它们的精子囊泡中，可能繁殖单向性。本研究的目的是揭示aspermic fasciola flukes的起源。核单拷贝标记物，磷酸丙酮酸羧基酶和DNA聚合酶，用于分析来自中国的诉讼。通过FH / FG型存在，强烈建议患有患有患有患有Fasciola Fluk的杂化起源，其包括F.Hepatica和F.Gigantica的DNA片段。中国可以被视为杂交的摇篮，因为在中国北部和南部地区检测到肝癌和F.Gigantica分别在两种物种的栖息地之间分配了杂交种群体。中国的来源得到了大量的线粒体Nadhdehydogens亚基1（NAD1）单倍型在中国曲囊血糖菌群中检测到，而不是邻国的aspermic群体。此后，'Aspermic'Fasciola Flukes应该被称为“杂交”敌胞胎氟尿嘧啶。

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