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首页> 外文期刊>Parasitology >Using MALDI-TOF MS to identify mosquitoes collected in Mali and their blood meals
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Using MALDI-TOF MS to identify mosquitoes collected in Mali and their blood meals

机译：使用MALDI-TOF MS来识别MALI和血液中收集的蚊子

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摘要

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. To test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of Mali. We successfully analysed the blood meals from 651 mosquito abdomens crushed on Whatman filter paper (WFPs) in the field using MALDI-TOF MS. The legs of 826 mosquitoes were then submitted for MALDI-TOF MS analysis in order to identify the different mosquito species. Eight mosquito species were identified, including Anopheles gambiae Giles, Anopheles coluzzii, Anopheles arabiensis, Culex quinquefasciatus, Culex neavei, Culex perexiguus, Aedes aegypti and Aedes fowleri in Mali. The field mosquitoes for which MALDI-TOF MS did not provide successful identification were not previously available in our database. These specimens were subsequently molecularly identified. The WFP blood meal sources found in this study were matched against human blood (n = 619), chicken blood (n = 9), cow blood (n = 9), donkey blood (n = 6), dog blood (n = 5) and sheep blood (n = 3). This study reinforces the fact that MALDI-TOF MS is a promising tool for entomological surveys.
机译：矩阵辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF MS）已被描述为识别节肢动物和蚊子血粕来源的创新有效工具。为了在该领域的昆虫学调查的背景下测试这种方法，从马里的五个生态上鲜明地区收集蚊子。我们成功地分析了使用MALDI-TOF MS在现场的Whatman滤纸（WFPS）上的651蚊子腹部的血粉。然后将826个蚊子的腿提交MARDI-TOF MS分析，以识别不同的蚊虫。鉴定了八种蚊虫种类，包括anopheles冈比亚吉尔斯，诺谢斯·罗利尼，anopheles Arabiensis，Culex Quinquefasciatus，Culex Neavei，Culex Perexiguus，Aedes Aegypti和Mali的Aedes Fowleri。 MALDI-TOF MS未提供成功识别的现场蚊子未在我们的数据库中提供。随后鉴定这些样品。本研究中发现的WFP血液膳食来源与人血（n = 619），鸡血（n = 9），牛血（n = 9），驴血（n = 6），狗血液（n = 5 ）和绵羊血液（n = 3）。本研究强化了MALDI-TOF MS是一个有希望的昆虫学调查的工具。

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  • 来源
    《Parasitology》 |2018年第9期|共13页
  • 作者

    Tandina Fatalmoudou; Niare Sirama; Laroche Maureen; Kone Abdoulaye K.; Diarra Adama Z.; Ongoiba Abdoulaye; Berenger Jean Michel; Doumbo Ogobara K.; Raoult Didier; Parola Philippe;

  • 作者单位

    Aix Marseille Univ IRD AP HM IHU Mediterranee Infect SSA VITROME Marseille France;

    Aix Marseille Univ IRD AP HM IHU Mediterranee Infect SSA VITROME Marseille France;

    Aix Marseille Univ IRD AP HM IHU Mediterranee Infect SSA VITROME Marseille France;

    Univ Sci Tech &

    Technol Bamako Malaria Res &

    Training Ctr Dept Epidemiol Parasit Dis Bamako Mali;

    Aix Marseille Univ IRD AP HM IHU Mediterranee Infect SSA VITROME Marseille France;

    Univ Sci Tech &

    Technol Bamako Malaria Res &

    Training Ctr Dept Epidemiol Parasit Dis Bamako Mali;

    Aix Marseille Univ IRD AP HM IHU Mediterranee Infect SSA VITROME Marseille France;

    Univ Sci Tech &

    Technol Bamako Malaria Res &

    Training Ctr Dept Epidemiol Parasit Dis Bamako Mali;

    Aix Marseille Univ IRD AP HM IHU Mediterranee Infect SSA VITROME Marseille France;

    Aix Marseille Univ IRD AP HM IHU Mediterranee Infect SSA VITROME Marseille France;

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  • 正文语种 eng
  • 中图分类 寄生虫病;
  • 关键词

    MALDI-TOF MS; mosquito; field; blood meals; Whatman; Mali;

    机译：MARDI-TOF MS;蚊子;场;血粉;Whatman;马里;

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