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首页> 外文期刊>Pest Management Science >Microbial HemG-type protoporphyrinogen IX oxidase enzymes for biotechnology applications in plant herbicide tolerance traits
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Microbial HemG-type protoporphyrinogen IX oxidase enzymes for biotechnology applications in plant herbicide tolerance traits

机译：用于生物技术应用在植物除草剂公差特征的生物技术应用的微生物Hemg型原子素IX氧化酶

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摘要

BACKGROUND Protoporphyrinogen IX oxidase (PPO)-inhibiting herbicides act by inhibiting a key enzyme in the heme and chlorophyll biosynthetic pathways in plants. This enzyme, the PPO enzyme, is conserved across plant species. However, some microbes are known to utilize a unique family of PPO enzymes, the HemG family. This enzyme family carries out the same enzymatic step as the plant PPO enzymes, but does not share sequence homology with the plant PPO enzymes. RESULTS Bioinformatic analysis was used to identify putative HemG PPO enzyme variants from microbial sources. A subset of these variants was cloned and characterized. HemG PPO variants were characterized for functionality and tolerance to PPO-inhibiting herbicides. HemG PPO variants that exhibited insensitivity to PPO-inhibiting herbicides were identified for further characterization. Expression of selected variants in maize, soybean, cotton and canola resulted in plants that displayed tolerance to applications of PPO-inhibiting herbicides. CONCLUSION Selected microbial-sourced HemG PPO enzyme variants present an opportunity for building new herbicide tolerance biotechnology traits. These traits provide tolerance to PPO-inhibiting herbicides and, therefore, could provide additional tools for farmers to employ in their weed management systems. (c) 2019 Society of Chemical Industry
机译：背景技术原因卟啉素IX氧化酶（PPO） - 通过抑制植物中血红素和叶绿素生物合成途径的关键酶来抑制除草剂。这种酶，PPO酶，在植物种类中保守。然而，已知一些微生物用于利用独特的PPO酶系列，HEMG系列。该酶家族作为植物PPO酶进行相同的酶促步骤，但不与植物PPO酶共享序列同源性。结果生物信息分析用于鉴定来自微生物来源的推定的HEMG PPO酶变体。将这些变体的子集克隆并表征。 HEMG PPO变体的特征是对抑制除草剂的功能性和耐受性。鉴定出表现出对PPO抑制除草剂的不敏感性的HEMG PPO变体用于进一步表征。玉米，大豆，棉花和油菜中所选变体的表达导致植物显示出PPO抑制除草剂的应用的耐受性。结论选定的微生物源性HEMG PPO酶变体为建立新的除草剂耐受生物技术特征提供了机会。这些特征为PPO抑制除草剂提供耐受性，因此可以为农民提供额外的工具，以便在其杂草管理系统中雇用。 （c）2019年化学工业协会

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