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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Dose dependent behavioral effects of acute alcohol administration in zebrafish fry
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Dose dependent behavioral effects of acute alcohol administration in zebrafish fry

机译：Zebrafish Fry中急性酒精管理的剂量依赖性行为效应

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The zebrafish is becoming increasingly utilized in behavioral neuroscience as it appears to strike a good compromise between practical simplicity and system complexity. Particularly in alcohol (ethanol) research, the zebrafish has been employed as a translationally relevant model organism. However, the majority of studies investigating the effects of alcohol on brain function and behavior has used adult zebrafish. In the current study, we utilize 6-8 post-fertilization day old larval zebrafish (fry) to investigate the effects of a 40 min-long, acute, immersion into the alcohol bath. We measure the behavioral responses of the fry during the immersion session in relatively large arenas, the petri dish, instead of the often employed 96 well plate, and report on significant modification of behavior induced by alcohol. For example, we found the intermediate dose of alcohol (0.5%, vol/vol) to exert a stimulant effect manifesting as slight elevation of swim speed, robust increase of turning, temporal variability of swim speed and turning, and diminished frequency of staying immobile. We also found the high dose of 1% alcohol to elicit an opposite response, a sedative effect. This biphasic dose response of alcohol mimics what has been found in mammals, including humans, and thus we conclude that a few day-old zebrafish fry may be a cost effective and efficient tool with which one can screen for small molecules or mutations with alcohol-effect modifying properties.
机译：斑马鱼越来越多地用于行为神经科学，因为它似乎在实际简单和系统复杂性之间取得了良好的折衷。特别是在醇（乙醇）研究中，斑马鱼已被雇用作为翻译相关的模型生物。然而，大多数研究调查酒精对脑功能和行为的影响使用了成年斑马鱼。在目前的研究中，我们利用6-8次施肥日龄幼虫斑马鱼（FRY）来研究40分钟，急性急剧浸入酒精浴中的影响。我们在相对较大的Arenas，培养皿中测量浸没期间Fry的行为响应，而不是经常使用的96孔板，并报告酒精诱导的行为的显着改性。例如，我们发现中间剂量的酒精（0.5％，Vol / Vol）发挥兴奋剂效果，表现为游泳速度的轻微升高，转弯的强大增加，游泳速度和转动的变化，以及保持不动的频率减少。我们还发现高剂量的1％酒精，以引发相反的反应，镇静作用。这种酒精模仿的双相剂量反应在包括人类的哺乳动物中发现了什么，因此我们得出结论，几天古老的斑马鱼油炸物可能是一种成本效益和有效的工具，其中一个人可以筛选小分子或用酒精的突变筛选效果修改属性。

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