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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Ropinirole protects against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced neurotoxicity in mice via anti-apoptotic mechanism.
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Ropinirole protects against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced neurotoxicity in mice via anti-apoptotic mechanism.

机译：通过抗凋亡机制，罗比尼罗基保护1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导小鼠的神经毒性。

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Ropinirole is a pharmacologically active agent used in the treatment of Parkinson's disease (PD) that directly acts on dopamine receptors. Recent studies reported that ropinirole has neuroprotective potential. However, there have been no detailed studies on apoptosis. Here we demonstrated the protective effects of ropinirole against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced neurotoxicity in a mouse model of PD. Unlike previous studies, we focused on apoptotic pathways. To confirm the protective effect of ropinirole, we conducted behavioral tests and tyrosine hydroxylase (TH)-immunohistochemistry. In the pole test and the rotarod test, commonly used behavioral tests in the mouse model of PD, ropinirole treatment (0.5, 1, or 2 mg/kg) maintained movement ability against MPTP-induced changes in motor coordination and postural balance, and bradykinesia. Our histological analyses illustrated that ropinirole significantly reduced MPTP-induced dopaminergic neuron damage in both the substantia nigra pars compacta (SNpc) and the striatum. In addition, we performed Western blot or kit analysis to measure apoptosis-related protein levels in the SNpc. Ropinirole increased the Bcl-2/Bax ratio, transcription factor A, and nuclear respiratory factor 1 and inhibited cytosolic cytochrome c release and caspase-3 activity, indicating that ropinirole inhibited the apoptotic cascade. These results suggest that ropinirole has neuroprotective effects in a mouse model of PD by inhibiting apoptosis.
机译：Ropinirole是一种药理学活性剂，用于治疗帕金森病（Pd）直接作用于多巴胺受体。最近的研究报告称Ropinirole具有神经保护潜力。但是，没有关于细胞凋亡的详细研究。在这里，我们证明了Ropinirole对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）的保护作用在Pd的小鼠模型中诱导的神经毒性。与以往的研究不同，我们专注于凋亡途径。为了确认罗比里尔的保护作用，我们进行了行为试验和酪氨酸羟化酶（Th）-immunohistochemisty。在杆试验和滚子测试中，在Pd的小鼠模型中常用的行为试验，罗比里尔治疗（0.5,1或2mg / kg）保持了对MPTP诱导的电动机协调和姿势平衡的变化的运动能力，以及Bradykinesia 。我们的组织学分析表明，Ropinirole显着降低了MPTP诱导的MPTP诱导的多巴胺能神经元损伤，在Implica NIGRA PARSCACTA（SNPC）和纹状体中。此外，我们进行了Western印迹或试剂盒分析，以测量SNPC中的细胞凋亡相关蛋白水平。罗比里洛增加了Bcl-2 / bax比，转录因子A和核呼吸系统1，抑制细胞溶质细胞色素C释放和Caspase-3活性，表明罗比里尔抑制了凋亡级联。这些结果表明，Ropinirole通过抑制细胞凋亡对PD的小鼠模型具有神经保护作用。

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