• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pharmacology, Biochemistry and Behavior >The effects of sub-anesthetic ketamine plus ethanol on behaviors and apoptosis in the prefrontal cortex and hippocampus of adolescent rats
【24h】

The effects of sub-anesthetic ketamine plus ethanol on behaviors and apoptosis in the prefrontal cortex and hippocampus of adolescent rats

机译：亚麻醉氯胺酮加乙醇对青少年大鼠前额外皮层和海马行为和凋亡的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ketamine has become increasingly popular in adolescent drug abusers worldwide. Meanwhile, alcohol is usually used by ketamine users. However, little work has been conducted to examine the chronic combined effects of ketamine and ethanol on adolescent brain. Here we probed into the effects of chronic administration of ketamine at recreational doses alone or combined with ethanol on behaviors and neuron damage in an adolescent rat model. 28-day old rats were treated with either 20 or 30 mg/kg ketamine plus or not plus 10% ethanol daily for 21 days. Depressive like behaviors, anxiety like behavior and memory impairment were tested using open field test, forced swimming test, elevated plus maze and Morris water maze. Apoptosis in prefrontal cortex (PFC) and hippocampus (HIP) were determined by the TdT-mediated dUTP Nick-End Labeling (TUNEL) and protein and mRNA levels of caspase-3, Bax and Bcl-2. Results show that co-application of ketamine and ethanol significantly increased immobility time in the forced swimming test, up-regulated TUNEL positive cells and both protein and mRNA expressions of caspase-3 and Bax, compared with the control group and ketamine and ethanol use alone groups in the PFC, but not in the HIP. Our study suggests that chronic co-administration of ketamine and ethanol results in depressive-like behavior and the caspase-dependent apoptosis in the PFC of adolescent rats' brains.
机译：氯胺酮在全世界的青少年吸毒者中越来越受欢迎。同时，酒精通常由氯胺酮使用者使用。然而，已经进行了很少的作品，以研究氯胺酮和乙醇对青少年脑的慢性组合作用。在这里，我们探讨了慢性酮酮在休闲剂量单独核施用或与乙醇联合在青少年大鼠模型中的行为和神经元损伤中。每日20或30mg / kg氯胺酮加或不加10％乙醇治疗28天的老鼠21天。使用开放式测试，强制游泳试验，高迷宫和莫里斯水迷宫测试了令人抑郁的行为，像行为和内存障碍等焦虑，焦虑是测试的。前额叶皮质（PFC）和海马（HIP）中的细胞凋亡由TDT介导的DUTP缩小末端标记（TUNEL）和蛋白质和mRNA水平和mRNA水平测定Caspase-3，Bax和Bcl-2。结果表明，与对照组和氯胺酮和乙醇单独使用，氯胺酮和乙醇的共同施加在强制游泳试验，上调调节试验，上调的调节阳性细胞和蛋白质和mRNA表达中显着增加了蛋白质-3和Bax的蛋白质和mRNA表达PFC中的群体，但不在臀部。我们的研究表明，慢性共同施用氯胺酮和乙醇导致抑郁样行为和青少年大鼠脑PFC中的依赖性凋亡。

著录项

  • 来源
    《Pharmacology, Biochemistry and Behavior》 |2019年第2019期|共11页
  • 作者

    Li Qing; Wu Hui-ran; Fan Shu-juan; Liu De-xiang; Jiang Hong; Zhang Qian; Pan Fang;

  • 作者单位

    Shandong Univ Sch Basic Med Sci Dept Med Psychol &

    Med Eth 44 Wenhua Xi Rd Jinan 250012;

    Shandong Univ Sch Basic Med Sci Dept Med Psychol &

    Med Eth 44 Wenhua Xi Rd Jinan 250012;

    Shandong Univ Sch Basic Med Sci Dept Med Psychol &

    Med Eth 44 Wenhua Xi Rd Jinan 250012;

    Shandong Univ Sch Basic Med Sci Dept Med Psychol &

    Med Eth 44 Wenhua Xi Rd Jinan 250012;

    Shandong Univ Sch Basic Med Sci Dept Med Psychol &

    Med Eth 44 Wenhua Xi Rd Jinan 250012;

    Shandong Univ Sch Basic Med Sci Dept Med Psychol &

    Med Eth 44 Wenhua Xi Rd Jinan 250012;

    Shandong Univ Sch Basic Med Sci Dept Med Psychol &

    Med Eth 44 Wenhua Xi Rd Jinan 250012;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药理学;
  • 关键词

    Apoptosis; Prefrontal cortex; Hippocampus; Ketamine; Abuse; Adolescent;

    机译：细胞凋亡;前额叶皮质;海马;氯胺酮;滥用;青少年;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号