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首页> 外文期刊>Physiologia plantarum >Desiccation-induced alterations in surface topography of thylakoids from resurrection plant Haberlea rhodopensis studied by atomic force microscopy, electrokinetic and optical measurements
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Desiccation-induced alterations in surface topography of thylakoids from resurrection plant Haberlea rhodopensis studied by atomic force microscopy, electrokinetic and optical measurements

机译：从原子力显微镜，电动和光学测量研究的复活植物Haberlea rhodopensis中的干燥诱导的囊盂表面形貌的改变

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摘要

With their ability to survive complete desiccation, resurrection plants are a suitable model system for studying the mechanisms of drought tolerance. In the present study, we investigated desiccation-induced alterations in surface topography of thylakoids isolated from well-hydrated, moderately dehydrated, severely desiccated and rehydrated Haberlea rhodopensis plants by means of atomic force microscopy (AFM), electrokinetic and optical measurements. According to our knowledge, so far, there were no reports on the characterization of surface topography and polydispersity of thylakoid membranes from resurrection plants using AFM and dynamic light scattering. To study the physicochemical properties of thylakoids from well-hydrated H. rhodopensis plants, we used spinach thylakoids for comparison as a classical model from higher plants. The thylakoids from well-hydrated H. rhodopensis had a grainy surface, significantly different from the well-structured spinach thylakoids with distinct grana and lamella, they had twice smaller cross-sectional area and were 1.5 times less voluminous than that of spinach. Significant differences in their physicochemical properties were observed. The dehydration and subsequent rehydration of plants affected the size, shape, morphology, roughness and therefore the structure of the studied thylakoids. Drought resulted in significant enhancement of negative charges on the outer surface of thylakoid membranes which correlated with the increased roughness of thylakoid surface. This enhancement in surface charge density could be due to the partial unstacking of thylakoids exposing more negatively charged groups from protein complexes on the membrane surface that prevent from possible aggregation upon drought stress.
机译：随着他们存活完全干燥的能力，复活植物是研究耐旱机制的合适模型系统。在本研究中，我们通过原子力显微镜（AFM），电动和光学测量，研究了从水性水解，中脱水，严重干燥的哈米卵氏菌植物中分离的类囊体的表面形貌的干燥诱导的改变。根据我们的知识，到目前为止，没有关于使用AFM和动态光散射从复活植物的表面形貌和囊状膜的多分散性的报告。为了研究来自水性水解的rhodopensis植物的囊体的物理化学特性，我们使用菠菜囊体作为高等植物的经典模型进行比较。来自水性水合水H. rhodopensis的粒状表面具有颗粒状表面，与具有不同瘙痒和薄片的结构良好的菠菜囊体显着不同，它们具有两倍的横截面积，并且比菠菜的较低的1.5倍。观察到其物理化学性质的显着差异。植物的脱水和随后的再水合影响了尺寸，形状，形态，粗糙度，因此是所研究的类囊体的结构。干旱导致囊体膜外表面上的负电荷显着提高，与囊泡表面的粗糙度增加相关。表面电荷密度的这种增强可能是由于囊突的部分未填充从膜表面上的蛋白质复合物暴露更多带负电荷的基团，其在干旱胁迫下防止可能的聚集。

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  • 来源
    《Physiologia plantarum》 |2019年第2期|共11页
  • 作者

    Doltchinkova Virjinia; Andreeva Tonya; Georgieva Katya; Mihailova Gergana; Balashev Konstantin;

  • 作者单位

    Sofia Univ St Kliment Ohridski Dept Biophys &

    Radiobiol Fac Biol Sofia 1164 Bulgaria;

    Bulgarian Acad Sci Inst Biophys &

    Biomed Engn BU-1113 Sofia Bulgaria;

    Bulgarian Acad Sci Inst Plant Physiol &

    Genet BU-1113 Sofia Bulgaria;

    Bulgarian Acad Sci Inst Plant Physiol &

    Genet BU-1113 Sofia Bulgaria;

    Sofia Univ St Kliment Ohridski Fac Chem &

    Pharm Dept Phys Chem Lab Biophys Chem Sofia 1164 Bulgaria;

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  • 正文语种 eng
  • 中图分类 植物生理学;
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