Silencing of glycerol-3-phosphate acyltransferase 6 (GPAT6) gene using a newly established virus induced gene silencing (VIGS) system in cucumber alleviates autotoxicity mimicked by cinnamic acid (CA)

Bu Ruifang; Wang Runhao; Wei Qichao; Hu Haiyan; Sun Haili; Song Puwen; Yu Yongang; Liu Qili; Zheng Zichao; Li Tong; Li Dongxiao; Wang Lei; Chen Shijun; Wu Liuliu; Wu Jianyu; Li Chengwei

首页> 外文期刊>Plant and Soil >Silencing of glycerol-3-phosphate acyltransferase 6 (GPAT6) gene using a newly established virus induced gene silencing (VIGS) system in cucumber alleviates autotoxicity mimicked by cinnamic acid (CA)

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Silencing of glycerol-3-phosphate acyltransferase 6 (GPAT6) gene using a newly established virus induced gene silencing (VIGS) system in cucumber alleviates autotoxicity mimicked by cinnamic acid (CA)

机译：使用新建立的病毒诱导基因沉默（VPPAT6）基因在黄瓜中沉默于黄瓜中的沉默可缓解由肉桂酸（CA）模仿的自诱毒性

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Background and aimsAutotoxicity is a serious problem for continuous cropping of cucumber, it can limit its own growth and that of nearby cucumber plants and decrease yield and quality. Cinnamic acid (CA) derivatives are a class of autotoxins that can mimic autotoxic effects in cucumber.MethodsWe screened an Arabidopsis long hairpin RNA (lhRNA) library under CA-mimicked autotoxicity stress to obtain autotoxicity-resistant mutant lines. The glycerol-3-phosphate 2-O-acyltransferase 6 (GPAT6) gene was found to be silenced in one resistant mutant line, and GPAT6 homologous gene in cucumber was cloned and named CuGPAT6. To test gene function quickly, a new tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) system was established in cucumber using a special agroinfiltration solution and whole cotyledonary nodes.ResultsCucumber phytoene desaturase gene (CuPDS) and CuGPAT6 gene were successfully silenced with a newly established tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) system in cucumber showing photobleaching and decreased CuPDS expression in leaves; and silencing of CuGPAT6 enhanced the tolerance to the autotoxin stress mimicked by CA in cucumber.ConclusionsIt indicated that the alleviation of the damage caused by CA treatment was probably through maintaining the integrity of cellular structure, supporting cell vitality and promoting growth in roots, and increasing the integrate chloroplast and thylakoid numbers in leaves, which restored the growth and net photosynthesis rate of CA treated CuGPAT6 silenced plants to the similar level to those of control plants. The results also demonstrated that the newly established TRV mediated VIGS system is functional, rapid, convenient and highly efficient in cucumber.

机译：背景和Aimsautotoxicity是对黄瓜连续种植的严重问题，它可以限制自己的增长和附近的黄瓜植物的增长，降低收益率和质量。肉桂酸（CA）衍生物是一类可以在Cucumber中模拟自诱毒性效应的一类。方法在Ca-模仿的自诱变应力下筛选拟南芥长发夹RNA（LHRNA）文库，以获得自诱导性抗性突变体。发现甘油-3-磷酸2-O-酰基转移酶6（GPAT6）基因在一种抗性突变体中沉默，并且黄瓜中的GPAT6同源基因被克隆并命名为Cugpat6。为了快速测试基因功能，使用特殊的农药滤液溶液和整个子叶植物节点在黄瓜中建立了新的烟草响病毒（TRV）介导的病毒诱导的基因沉默（Vigs）系统。培养番茄植物植物基因（CUPDS）和Cugpat6基因，在黄瓜中建立了黄瓜。成功地沉默于新建立的烟草响病毒（TRV）介绍的病毒诱导的基因沉默（Vigs）系统，在黄瓜中显示出光博展示和叶子中的Cupds表达减少; Cugpat6的沉默增强了由CaIn在黄瓜中的Ca模仿的自诱毒性应力的耐受性。结论表明，通过维持细胞结构的完整性，促进细胞生命力和促进根部生长的完整性，以及促进根部的生长，以及增加的损害叶片中的整合叶绿体和囊体数，恢复了Ca治疗的Cugpat6沉默植物的生长和净光合速率与对照植物的那些。结果还表明，新建立的TRV介导的Vigs系统在黄瓜中具有功能性，快速，方便，高效，高效。

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来源
《Plant and Soil》 |2019年第2期|共18页
作者
Bu Ruifang; Wang Runhao; Wei Qichao; Hu Haiyan; Sun Haili; Song Puwen; Yu Yongang; Liu Qili; Zheng Zichao; Li Tong; Li Dongxiao; Wang Lei; Chen Shijun; Wu Liuliu; Wu Jianyu; Li Chengwei;
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作者单位

Henan Agr Univ Coll Life Sci Zhengzhou 450000 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Chinese Acad Agr Sci Biotechnol Res Inst Beijing 100081 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

Henan Agr Univ Coll Life Sci Zhengzhou 450000 Peoples R China;

Henan Inst Sci &

Technol Coll Life Sci &

Technol Henan Engn Res Ctr Crop Genome Editing Xinxiang 453003 Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类土壤学;
关键词
Cucumber; Autotoxicity; Arabidopsis lhRNA library; GPAT6; TRV mediated VIGS; Agroinfiltration;

机译：黄瓜;自诱毒性;Arabidopsis lhrna图书馆;GPAT6;TRV介导的Vigs;农药过滤;

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专利

1. Silencing of glycerol-3-phosphate acyltransferase 6 (GPAT6) gene using a newly established virus induced gene silencing (VIGS) system in cucumber alleviates autotoxicity mimicked by cinnamic acid (CA) [J] . Bu Ruifang, Wang Runhao, Wei Qichao, Plant and Soil . 2019,第1a2期

机译：使用新建立的病毒诱导基因沉默（VPPAT6）基因在黄瓜中沉默于黄瓜中的沉默可缓解由肉桂酸（CA）模仿的自诱毒性
2. Virus-induced gene silencing (VIGS) in Lilium leichtlinii using the Cucumber mosaic virus vector [J] . Keisuke Tasaki, Hiroyuki Terada, Chikara Masuta, Plant Biotechnology . 2016,第5期

机译：黄瓜花叶病毒载体在百合中的病毒诱导基因沉默（VIGS）
3. Virus-induced gene silencing (VIGS) in Lilium leichtlinii using the Cucumber mosaic virus vector [J] . Plant Biotechnology . 2016,第5期

机译：使用黄瓜马赛克病毒载体的病毒诱导的基因沉默（Vigs）在Lilium Leichtlinii中
4. Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes [C] . Meng Ma, Huixian Zhao International gluten workshop;Ministry of Agriculture;Ministry of Science and Technology;Studies of Wheat Agronomic Traits and Varietal Improvement . 2012

机译：小麦穗粒中病毒诱导的基因沉默及其在HMW-GS编码基因功能分析中的应用
5. Control of Plant Meiosis Using Virus-Induced Gene Silencing (VIGS) [D] . Calvo-Baltana?s, Vanesa. 2019

机译：使用病毒诱导的基因沉默控制植物减数分裂（Vigs）
6. Virus-induced gene silencing (VIGS) in Lilium leichtlinii using the Cucumber mosaic virus vector [O] . Keisuke Tasaki, Hiroyuki Terada, Chikara Masuta, 2016

机译：黄瓜花叶病毒载体在百合中的病毒诱导基因沉默（VIGS）
7. Transcriptome Analysis of Rpp4 Silenced Plants via virus-induced gene silencing (VIGS). [O] . MORALES A. M. A. P., PEREIRA A. A., LINCOLN L., 2012

机译：通过病毒诱导的基因沉默（VIGS）对Rpp4沉默植物进行转录组分析。

Silencing of glycerol-3-phosphate acyltransferase 6 (GPAT6) gene using a newly established virus induced gene silencing (VIGS) system in cucumber alleviates autotoxicity mimicked by cinnamic acid (CA)

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