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首页> 外文期刊>Plant and Soil >Expression analysis of calcium-dependent protein kinases (CDPKs) superfamily genes in Medicago lupulina in response to high calcium, carbonate and drought
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Expression analysis of calcium-dependent protein kinases (CDPKs) superfamily genes in Medicago lupulina in response to high calcium, carbonate and drought

机译：Medicago Lupulina中钙依赖性蛋白激酶（CDPKS）超家族基因的表达分析响应高钙，碳酸盐和干旱

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摘要

Aims The aim of this study is to identify calcium dependent protein kinases (CDPKs) superfamily genes (including calcium dependent protein kinase (CDPK) genes, CDPK-related kinase (CRK) genes, and phosphoenolpyruvate carboxylase kinase-related kinase (PEPRK) genes) and to analyze their expression in response to high calcium, bicarbonate, and water deficit in Medicago lupulina. Methods Transcriptome sequencing databases and bioinformatics were used to identify CDPK superfamily genes. qRT-PCR analyses were used to study the expression of CDPK superfamily genes in response to high calcium, bicarbonate, water deficit, and their combined treatments. Results Ten MlCDPKs, four MlCRKs, and two MlPEPRKs genes were identified from transcriptome sequencing databases. The identified MlCDPK superfamily genes could be divided into six subgroups including CDPK I, CDPK II, CDPK III, CDPK IV, CRK, and PEPRK according to evolutionary relationships. Analysis of protein structure indicated that MlCDPK proteins contained a protein kinase domain and EF-hand domain. However, PEPRKs contained only a protein kinase domain while CRKs contained also degenerative EF-hands. Most of the MlCDPK superfamily genes were differentially expressed in different organs. According to their expression levels, MlCDPK superfamily genes such as MlCDPK1, MlCDPK9, and MlCDPK10 were more sensitive in response to individual treatments of high calcium, carbonate or PEG rather than their combination of treatments. The results indicated that MlCDPK superfamily genes may play an important role in response to those stresses. Conclusions These results provide important insight into the role of CDPKs in M. lupulina, especially in the tolerance to Karst habitats, where high calcium and carbonate and drought conditions are the dominant ecological limiting factors.
机译：本研究的目的是鉴定钙依赖性蛋白激酶（CDPK）超家族基因（包括钙依赖性蛋白激酶（CDPK）基因，CDPK相关激酶（CRK）基因和磷酸丙酮属羧化酶激酶相关激酶相关激酶（PEPRK）基因）并响应高钙，碳酸氢盐和Medicago Lupulina的水赤字分析它们的表达。方法使用转录组测序数据库和生物信息学用于鉴定CDPK超家族基因。使用QRT-PCR分析来研究CDPK超家族基因响应高钙，碳酸氢盐，水缺损及其组合治疗的表达。结果，从转录组测序数据库中鉴定出十MLCDPKS，四个MLCRK和两种MLPeprks基因。鉴定的MLCDPK超家族基因可以分为六个亚组，包括CDPK I，CDPK II，CDPK III，CDPK IV，CRK和PEPRK，根据进化关系。蛋白质结构分析表明MLCDPK蛋白含有蛋白激酶结构域和EF手结构域。然而，PEPRKS仅包含蛋白激酶域，而扣篮也含有退行性EF手。大多数MLCDPK超家族基因在不同的器官中差异表达。根据其表达水平，MLCDPK超家族基因如MLCDPK1，MLCDPK9和MLCDPK10响应于高钙，碳酸盐或PEG的个体处理而不是它们的治疗组合更敏感。结果表明，MLCDPK超家族基因可能在响应那些应力时发挥重要作用。结论这些结果对CDPKS在M. Lupulina的作用，特别是在对喀斯特栖息地的耐受性方面提供了重要的洞察力，其中高钙和碳酸盐和干旱条件是主要的生态限制因素。

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  • 来源
    《Plant and Soil》 |2019年第2期|共16页
  • 作者

    Zhang Xi-Min; Liu Lun-Xian; Su Zhi-Meng; Tang Jing; Shen Zhi-Jun; Gao Gui-Feng; Yi Yin; Zheng Hai-Lei;

  • 作者单位

    Xiamen Univ Coll Environm &

    Ecol Key Lab Subtrop Wetland Ecosyst Res MOE Xiamen 361102 Fujian Peoples R China;

    Guizhou Normal Univ Key Lab Plant Physiol &

    Dev Regulat Key Lab Biodivers Conservat Karst Mt Area Southwe Forestry Minist China Sch Life Sci Guiyang 550001 Guizhou Peoples R China;

    Guizhou Normal Univ Key Lab Plant Physiol &

    Dev Regulat Key Lab Biodivers Conservat Karst Mt Area Southwe Forestry Minist China Sch Life Sci Guiyang 550001 Guizhou Peoples R China;

    Guizhou Normal Univ Key Lab Plant Physiol &

    Dev Regulat Key Lab Biodivers Conservat Karst Mt Area Southwe Forestry Minist China Sch Life Sci Guiyang 550001 Guizhou Peoples R China;

    Xiamen Univ Coll Environm &

    Ecol Key Lab Subtrop Wetland Ecosyst Res MOE Xiamen 361102 Fujian Peoples R China;

    Xiamen Univ Coll Environm &

    Ecol Key Lab Subtrop Wetland Ecosyst Res MOE Xiamen 361102 Fujian Peoples R China;

    Guizhou Normal Univ Key Lab Plant Physiol &

    Dev Regulat Key Lab Biodivers Conservat Karst Mt Area Southwe Forestry Minist China Sch Life Sci Guiyang 550001 Guizhou Peoples R China;

    Xiamen Univ Coll Environm &

    Ecol Key Lab Subtrop Wetland Ecosyst Res MOE Xiamen 361102 Fujian Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 土壤学;
  • 关键词

    Medicago lupulina; Calcium signaling; CDPKs; Phylogenetic analysis; Karst habitat;

    机译：Medicago lucepuline;钙信号;CDPKS;哲学分析;KST栖息地;

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