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首页> 外文期刊>Plant Cell Reports >Ectopic expression of LoSVP, a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis
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Ectopic expression of LoSVP, a MADS-domain transcription factor from lily, leads to delayed flowering in transgenic Arabidopsis

机译：LOSV的异位表达，百合的疯子域转录因子，导致转基因拟南芥的开花延迟

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摘要

Key message A MADS-domain transcription factorLoSVP, which could delay flowering through vernalization pathway, was isolated from lily. MADS-domain transcription factors play important roles in plant growth and development, especially in the transition from vegetative phase to reproductive phase. However, their functions in bulbous flowering plants are largely unknown. In this work, a SHORT VEGETATIVE PHASE (SVP) encoding genes LoSVP from oriental lily was isolated. Bioinformatic analyses demonstrated that LoSVP encodes a type II MADS-box protein containing a conserved MADS-box, as well as a conserved K-box domain. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed ubiquitous expression of LoSVP in various tissues, including petals, stamens, pistils, leaves and scales. Real-time polymerase chain reaction (PCR) analyses demonstrated that LoSVP was predominantly expressed in the early stage of developing flowers. Constitutive expression of LoSVP in Arabidopsis led to significantly delayed flowering of transgenic plants. These results suggest that LoSVP is involved in plant flowering and could be used as a potential candidate gene for the genetic regulation of flowering time in higher plants.
机译：关键消息是可以通过富含vernalization途径延迟开花的疯狂域转录因子，从百合中分离出来。 MADS-域转录因子在植物生长和发展中发挥着重要作用，特别是在从营养阶段转变为生殖阶段。然而，它们在球根开花植物中的功能在很大程度上是未知的。在这项工作中，分离了从东方百合中编码了洛杉矶基因的短暂营养阶段（SVP）。生物信息分析表明，LOSVP编码了含有保守疯盒的II型疯子箱蛋白，以及保守的K盒领域。半定量逆转录聚合酶链反应（RT-PCR）揭示了各种组织中LOSV的普遍存在的表达，包括花瓣，雄蕊，雌蕊，叶和鳞片。实时聚合酶链式反应（PCR）分析表明，LOSVP主要表达在养殖花的早期阶段。拟南芥LOSVP的组成型表达导致转基因植物的显着延迟开花。这些结果表明LOSVP参与植物开花，可用作潜在的候选基因，用于高等植物中开花时间的遗传调节。

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  • 来源
    《Plant Cell Reports》 |2020年第2期|共10页
  • 作者

    Tang Xiaoli; Liang Meixia; Han Junjie; Cheng Jieshan; Zhang Hongxia; Liu Xiaohua;

  • 作者单位

    Ludong Univ Coll Agr 186 Hongqizhong Rd Yantai 264025 Shandong Peoples R China;

    Ludong Univ Coll Agr 186 Hongqizhong Rd Yantai 264025 Shandong Peoples R China;

    Yantai Acad Agr Sci 26 West Gangcheng St Yantai 265500 Shandong Peoples R China;

    Ludong Univ Coll Agr 186 Hongqizhong Rd Yantai 264025 Shandong Peoples R China;

    Ludong Univ Coll Agr 186 Hongqizhong Rd Yantai 264025 Shandong Peoples R China;

    Ludong Univ Coll Agr 186 Hongqizhong Rd Yantai 264025 Shandong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物细胞学;
  • 关键词

    MADS-box; Lily; LoSVP; Transgenic plant;

    机译：疯了;百合;LOSVP;转基因植物;

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