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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Characterization and quantification of flavonoids and organic acids over fruit development in American cranberry (Vaccinium macrocarpon) cultivars using HPLC and APCI-MS/MS
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Characterization and quantification of flavonoids and organic acids over fruit development in American cranberry (Vaccinium macrocarpon) cultivars using HPLC and APCI-MS/MS

机译:使用HPLC和APCI-MS / MS在美国蔓越莓(髋臼炔烃类)品种果实中黄酮类化合物和有机酸的表征及定量

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摘要

Cranberry flavonoids, including anthocyanins, flavonol glycosides and proanthocyanidins, and organic acids were characterized and quantified by HPLC and LC-MS/MS during fruit development and ripening in eight cranberry cultivars. Anthocyanin biosynthesis initiated at early fruit development and reached highest level in mature fruit, with significant differences between cultivars. Major flavonol glycosides, including the most abundant quercetin-3-galactoside and myricetin-3-galactoside, showed consistent concentrations during the season with moderate fluctuation, and were at similar levels in mature fruits of the eight cultivars. Proanthocyanidins declined during fruit development and then increased slightly in later maturation stages. Levels of various proanthocyanidin oligomers/polymers with different degree-of-polymerization were highly correlated within a cultivar during fruit development. Cultivars with coancestry exhibited similar levels (high/low) of anthocyanins or proanthocyanidins, indicating genetic effects on biosynthesis of such flavonoids. All cultivars showed similar levels of malic and citric acids, and declining levels of quinic acid during fruit development. Benzoic acid was extremely low early in the season and increased sharply during fruit ripening. Levels of quinic and citric acids were significantly different among cultivars in the mature fruit. Concentrations of proanthocyanidins, anthocyanins, quinic acid and benzoic acid have a strong developmental association in developing ovaries.
机译:在果实发育期间,通过HPLC和LC-MS / MS在果实发育过程中表征和量化蔓越莓黄酮类,包括花青素,黄酮醇糖苷和原花青素和有机酸。花青素生物合成在早期果实发育中发起,达到成熟果实的最高水平,品种之间具有显着差异。主要的黄酮醇糖苷,包括最丰富的槲皮素-3-半乳糖糖苷和霉菌蛋白-3-半乳糖糖苷,在季节表现出一致的浓度,中等波动,并且在八种品种的成熟果实中处于类似的水平。花青霉素在果实发育过程中下降,然后在后来的成熟阶段略微增加。具有不同聚合程度的各种花青素蛋白的水平/聚合物在果实发育过程中的栽培品种中高度相关。具有同步的栽培品种表现出类似的水分(高/低)的花青素或花青蛋白,表明这种类黄酮的生物合成的遗传作用。所有品种均显示出类似水平的苹果酸和柠檬酸,以及在水果发育过程中降低奎酸水平。在季节早期苯甲酸极低,在果实成熟过程中急剧增加。成熟果实中的品种中奎尼和柠檬酸的水平显着差异。花青素,花青素,醋酸和苯甲酸的浓度在显影卵巢中具有良好的发育联系。

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