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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Co-overexpression of AVP1 and PP2A-C5 in Arabidopsis makes plants tolerant to multiple abiotic stresses
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Co-overexpression of AVP1 and PP2A-C5 in Arabidopsis makes plants tolerant to multiple abiotic stresses

机译：AVP1和PP2A-C5在拟南芥中的共同表达使植物耐受多种非生物应激

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摘要

Abiotic stresses are major threats to agricultural production. Drought and salinity as two of the major abiotic stresses cause billions of losses in agricultural productivity worldwide each year. Thus, it is imperative to make crops more tolerant. Overexpression of AVP1 or PP2A-C5 was previously shown to increase drought and salt stress tolerance, respectively, in transgenic plants. In this study, the hypothesis that co-overexpression of AVP1 and PP2A-C5 would combine their respective benefits and further improve salt tolerance was tested. The two genes were inserted into the same T-DNA region of the binary vector and then introduced into the Arabidopsis genome through Agrobacterium-mediated transformation. Transgenic Arabidopsis plants expressing both AVP1 and PP2A-C5 at relatively high levels were identified and analyzed. These plants displayed enhanced tolerance to NaCl compared to either AVP1 or PP2A-C5 overexpressing plants. They also showed tolerance to other stresses such as KNO3 and LiCl at harmful concentrations, drought, and phosphorus deficiency at comparable levels with either AVP1 or PP2A-C5 overexpressing plants. This study demonstrates that introducing multiple genes in single T-DNA region is an effective approach to create transgenic plants with enhanced tolerance to multiple stresses.
机译：非生物胁迫是对农业生产的主要威胁。作为两个主要的非生物胁迫的干旱和盐度，每年在全球范围内导致农业生产力的数十亿亏损。因此，必须使农作物更容许耐受性。预先表达AVP1或PP2A-C5的过表达分别在转基因植物中增加了干旱和盐胁迫耐受性。在该研究中，AVP1和PP2A-C5共同过表达的假设将结合各自的益处，并进一步改善耐盐性。将两个基因插入二元载体的相同T-DNA区域中，然后通过农杆菌介导的转化引入拟南芥基因组中。鉴定并分析表达在相对高水平的AVP1和PP2A-C5的转基因拟南芥植物。与AVP1或PP2A-C5过表达植物相比，这些植物对NaCl的耐受性增强了耐受性。它们还向其他应力（如KNO3和LICL）的耐受性，干旱和PP2A-C5过表达植物的耐受浓度，干旱和磷缺乏的耐受性浓度，干旱和磷缺乏。该研究表明，在单个T-DNA区域引入多种基因是一种有效的方法，以产生具有增强的对多重应力的转基因植物。

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