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首页> 外文期刊>Plant Pathology >IGS region polymorphisms are responsible for failure of commonly used species-specific primers in Fusarium proliferatum isolates from diseased garlic
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IGS region polymorphisms are responsible for failure of commonly used species-specific primers in Fusarium proliferatum isolates from diseased garlic

机译：IGS区多态性是患有患病大蒜的镰刀菌常用物种特异性引物的常用物种特异性引物的失效

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摘要

Fusarium proliferatum is a globally distributed fungal pathogen that affects a range of crop hosts and is one of the main producers of mycotoxins, such as fumonisins, in foods. Specific PCR primers are commonly used for detection and identification of this pathogen. The aim of this study was to validate previously published F. proliferatum-specific primers targeting the intergenic spacer (IGS) region and characterize intraspecific variation and homologous recombination events for isolates obtained from diseased garlic bulbs in Spain. Sixty-nine isolates were morphologically identified as F. proliferatum, and their identity was confirmed by sequencing of the translation elongation factor; however, specific IGS primers did not result in an amplification product for nine of these isolates. Further analysis showed that this was due to polymorphism in the IGS region and six isolates were classified as IGS type I, while the remaining isolates were type II. Sequencing of the complete IGS region revealed numerous sequence polymorphisms amongst F. proliferatum isolates, and regions of recombination. Duplication and deletion events may have occurred via unequal crossing over during mitotic or meiotic recombination. These results suggest that the IGS region may be too variable as a reliable target for F. proliferatum-specific identification.
机译：Fusarium proLiferatum是一种全球分布的真菌病原体，影响一系列作物宿主，是食品中霉菌毒素的主要生产商之一。特异性PCR引物通常用于检测和鉴定该病原体。该研究的目的是验证以前公开的F.靶向代骨间隔区（IGS）区域的增殖性特异性引物，并表征了从西班牙患病的大蒜鳞茎获得的分离株的分离物的内部变化和同源重组事件。将六十九分离物形态学鉴定为F.增殖，并通过对翻译伸长因子的测序确认它们的身份;然而，特异性IgS引物未导致九个分离株的扩增产物。进一步的分析表明，由于IGS区域中的多态性，并且将六个分离株分类为IGS I型，而剩余的分离物是II型。完整IGS区域的测序显示了含有F.增殖性分离物中的许多序列多态性和重组区域。在有丝分裂或减数分裂重组期间，可以通过不等交叉发生复制和缺失事件。这些结果表明IgS区域可能太可变为太可变量，作为Proviferatum特异性鉴定的可靠靶标。

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