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首页> 外文期刊>Plant Pathology >Heterodera schachtii infection affects nitrogen metabolism in Arabidopsis thaliana
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Heterodera schachtii infection affects nitrogen metabolism in Arabidopsis thaliana

机译：heterodera schachtii感染影响拟南芥氮素代谢

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Nitrogen metabolism is important for physiological processes during normal growth and development, as well as plant defence responses. Second-stage juveniles of the cyst nematode Heterodera schachtii penetrate into the root and initiate permanent feeding sites called syncytia, from which they draw all necessary nutrients. To unravel whether the cyst nematode infestation changes nitrogen metabolism, the enzymatic activity and gene expression of nitrate (NIA) and nitrite (NIR) reductases and glutamate dehydrogenase (GDH), as well as levels of the metabolites nitrates, nitrites, and ammonium ions were estimated both locally in inoculated roots and systemically in shoots of inoculated Arabidopsis thaliana plants at 3, 7, and 15 days post-inoculation (dpi). Moreover, gene expression of long hypocotyle 5 (HY5), a basic-region leucine zipper (bZIP) transcription factor, was also determined, to learn more about the potential transcriptional regulation of the analysed enzymes. It was shown that the activity of nitrogen-related enzymes and the content of metabolites fluctuated during the whole period of infection. These results were accompanied by organ-specific and dpi-dependent gene expression patterns. The high gene expression level of HY5 in infected roots at 7 and 15 dpi coexisted with enhanced expression of NIA1 and NIR1 at 7 dpi and NIA2 at 15 dpi. Enhanced HY5 expression level in shoots of infected plants at 15 dpi was followed by up-regulation of NIA1 expression, suggesting that HY5 may regulate the gene expression of NIA and NIR during nematode infection. Our results indicate that changes in plant nitrogen metabolism occur during cyst nematode infection.
机译：在正常生长和发展期间，氮代谢对于生理过程以及植物防御反应是重要的。囊肿的二阶幼儿杂交杂交Schachtii渗透到根部并引发称为Syncytia的永久喂养部位，从中吸取所有必要的营养素。为了解开囊肿线虫侵扰的胚胎代谢，硝酸盐（Nia）和亚硝酸盐（NIR）还原酶和谷氨酸脱氢酶（GDH）的酶活性和基因表达，以及代谢物，亚硝酸盐和铵离子的水平在接种后3,7和15天的接种根部局部地接种的根部和系统性地，在接种后的3,7和15天的接种拟南芥植物中。此外，还确定了长次间隙5（HY5），碱性亮氨酸拉链（BZIP）转录因子的基因表达，以了解有关分析的酶的潜在转录调节的更多信息。结果表明，在整个感染期间，氮素相关酶的活性和代谢物的含量波动。这些结果伴有器官特异性和DPI依赖性基因表达模式。在7和15dpi的感染根中Hy5的高基因表达水平在15dpi下以7dpi和Nia2在7dpi和Nia 2的增强表达中共存。在15dPI的感染植物芽中的增强型Hy5表达水平随后是NIA1表达的上调，表明HY5可以调节NEMATODE感染期间NIA和NIR的基因表达。我们的结果表明，在囊肿的线虫感染期间发生植物氮代谢的变化。

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