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首页> 外文期刊>Physiology International: Acta Physiologica Hungarica >Curcumin regulates intracellular calcium release and inhibits oxidative stress parameters, VEGF, and caspase-3/-9 levels in human retinal pigment epithelium cells
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Curcumin regulates intracellular calcium release and inhibits oxidative stress parameters, VEGF, and caspase-3/-9 levels in human retinal pigment epithelium cells

机译：姜黄素调节细胞内钙释放并抑制人视网膜色素上皮细胞中的氧化应激参数，VEGF和Caspase-3 / -9水平

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摘要

In this study, we aimed to observe whether curcumin (cur), a polyphenolic compound derived from the dietary spice turmeric, a yellow substance obtained from the root of the plant Curcuma longa Linn, has any protective effect against blue light irradiation in human retinal pigment epithelium (ARPE-19) cells. For this purpose, we evaluated the intracellular calcium release mechanism, poly ADP ribose polymerase ( PARP), procaspase-3/-9 protein expression levels, caspase activation, and reactive oxygen species levels. ARPE-19 cells were divided into four main groups, such as control, cur, blue light, and cur + blue light. Results were evaluated by Kruskal-Wallis and Mann-Whitney U tests as post hoc tests. The cells in cur and cur + blue light samples were incubated with 20 mu M cur. Blue light exposure was performed for 24 h in an incubator. Lipid peroxidation and cytosolic-free Ca2+ [Ca2+](i) concentrations were higher in the blue light exposure samples than in the control samples; however, their levels were determined as significantly lower in the cur and cur + blue light exposure samples than in the blue light samples alone. PARP and procaspase-3 levels were significantly higher in blue light samples. Cur administration significantly decreased PARP and procaspase-3 expression levels. Reduced glutathione and glutathione peroxidase values were lower in the blue light exposure samples, although they were higher in the cur and cur + blue light exposure samples. Caspase-3 and -9 activities were lower in the cur samples than in the blue light samples. Moreover, vascular endothelial growth factor ( VEGF) levels were significantly higher in the blue light exposure samples. In conclusion, cur strongly induced regulatory effects on oxidative stress, intracellular Ca2+ levels, VEGF levels, PARP expression levels, and caspase-3 and -9 values in an experimental oxidative stress model in ARPE-19 cells.
机译：在这项研究中，我们旨在观察姜黄素（CUR），衍生自膳食香料姜黄的多酚化合物，从植物姜黄根Longa Linn的根部获得的黄色物质，对人视网膜颜料的蓝光照射具有任何保护作用上皮（ARPE-19）细胞。为此目的，我们评估了细胞内钙释放机制，聚ADP核糖聚合酶（PARP），Procaspase-3 / -9蛋白表达水平，胱天蛋酶活化和反应性氧物质水平。 ARPE-19细胞分为四个主要组，例如对照，Cur，蓝光和Cur +蓝光。结果由Kruskal-Wallis和Mann-Whitney U测试评估为后HOC测试。将Cur和Cur +蓝光样品中的细胞与20μmcur孵育。在培养箱中进行24小时进行蓝光暴露。蓝光暴露样品中的脂质过氧化和无核酸Ca2 + [Ca2 +]（I）浓度高于对照样品;然而，它们的水平在Cur和Cur +蓝光暴露样品中被确定为显着低于单独的蓝光样品。 PARP和Procaspase-3水平在蓝光样本中显着高。 Cur施用显着降低了PARP和ProCaspase-3表达水平。在蓝色曝光样品中，降低的谷胱甘肽和谷胱甘肽过氧化物酶值较低，但在Cur和Cur +蓝光暴露样品中较高。 Caspase-3和-9活性在Cur样品中较低，而不是蓝光样品。此外，蓝光暴露样品中的血管内皮生长因子（VEGF）水平显着高。总之，Cur强烈诱导ARPE-19细胞实验氧化应激模型中的氧化应激，细胞内Ca2 +水平，VEGF水平，PARP表达水平和Caspase-3和-9值的调节作用。

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