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首页> 外文期刊>Polymers for advanced technologies >Post‐polymerization modification of a new reactive monolith for reversed phase and hydrophilic interaction capillary electrochromatography of neutral, polar, and biologically active compounds
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Post‐polymerization modification of a new reactive monolith for reversed phase and hydrophilic interaction capillary electrochromatography of neutral, polar, and biologically active compounds

机译：用于反相和亲水性相互作用毛细管电粒度的新反相和生物活性化合物的新反相和亲水性相互作用的后聚合改性

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> A reactive monolith based on the polymerization of 3‐chloro‐2‐hydroxypropyl methacrylate, (HPMA‐Cl), with a crosslinking agent, ethylene glycol dimethacrylate (EDMA), was synthesized and post‐functionalized with a macromolecular ligand polyethyleneimine. Monolithic columns with controlled permeability and pore structure were prepared by free radical polymerization in the presence of a binary porogenic mixture of isopropanol and decanol. The presence of chloropropyl functionality in the pristine monolith allowed the synthesis of a post‐fuctionalized monolith carrying cationic groups that was used to control the magnitude of electroosmotic flow (EOF) in electrochromatographic separation. In the synthesis of pristine monoliths, the feed concentration of functional monomer (ie, HPMA‐Cl) was changed between 30 and 60? v /v % for obtaining cationic monoliths providing satisfactory electrochromatographic separation. The best electrochromatographic performance was obtained with the polyethyleneimine functionalized monolith prepared by using the pristine monolith obtained by 60% ( v /v) monomer concentration. This monolith was used in reversed phase and hydrophilic interaction capillary electrochromatography modes for the separation of alkylbenzenes, polycyclic aromatic hydrocarbons, phenols, and nucleosides, using mobile phases with low acetonitrile (ACN) contents ranging between 20% and 35% ( v /v). This ACN range was remarkably lower than the content of ACN used on the hydrophilic polymethacrylate‐based monoliths reported previously (ie, 90%). The plate heights up to 5.3?μm were obtained for the separation of nucleosides with the environmental friendly mobile phases whose ACN contents were also remarkably lower than that of similar polymethacrylate‐based monoliths.
机译： > 基于3-氯-2-羟丙基甲基丙烯酸甲酯（HPMA-CL）的聚合的反应性整料用交联剂，乙二醇二甲基丙烯酸酯（EDMA）合成，并用大分子配体聚乙烯亚胺后官能化。具有受控渗透性和孔结构的单片柱通过自由基聚合在异丙醇和癸醇的二元致致疣混合物存在下制备。原始整料中的氯丙基官能团的存在允许合成携带阳离子基团的延髓型整体，用于控制电杂色分离中的电渗流（EOF）的大小。在原始整料的合成中，官能单体（即HPMA-CL）的进料浓度在30和60之间变化？ v / v％用于获得提供令人满意的电杂色分离的阳离子单片。通过使用通过60％获得的原始单金属制备的聚乙烯亚胺官能化的整料（ v / v）单体浓度。该整料用于反相和亲水性相互作用毛细管电粒子缩影模式，用于分离烷基苯，多环芳烃，酚类和核苷，使用具有低乙腈（ACN）含量的流动相20％至35％（ v / v）。该ACN范围显着低于在先前报道的基于亲水性聚甲基丙烯酸甲酯的整体上使用的ACN的含量（即，＆ 90％）。获得高达5.3≤μm的板高度，用于将核苷与环含量的环含量分离，其ACN含量也显着低于与相似的聚甲基丙烯酸酯基整料。

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