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The therapeutic effect of Yinhuangerchen mixture on Avian infectious laryngotracheitis

机译：云康氏菌对禽传染性喉科炎的治疗作用

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摘要

The study was conducted to investigate the effect of Yinhuangerchen mixture (YM) on Avian infectious laryngotracheitis (AILT) induced by artificial infection and provide a scientific basis for its clinical application. A total of 200 chickens were challenged with infectious laryngotracheitis virus (ILTV). At 72 h post-challenge, the chickens were treated with different doses of YM or the Chinese herbal medicine Houyanjing powder. The relative expression of ILTV, the pathological changes of trachea, and the number of SIgA-secreting cells were detected. Thin-layer chromatography results confirmed that the YM contained Scutellaria baicalensis, Flos lonicerae, Pericarpium citri reticulatae, and Liquorice. The AILT model was successfully established by artificial infection. In the high-dose YM group (HD) and middle-dose YM group (MD), the effective rate of treatment was 100 and 96.7%, respectively, and the overall cure rate was 83.3%. In addition, the results of necropsy showed that the degree of tissue damage in chicken trachea was relatively low. Compared with positive control group, HD and MD chicken had lower relative expression of ILTV but more SIgA-secreting cells. In conclusion, YM can reduce ILTV level in tissue, mitigate tissue damage caused by infection, and enhance mucosal immunity having obvious therapeutic effect on AILT.
机译：进行该研究以研究人工感染诱导的云南混合物（YM）对禽类传染性喉部阴影炎（阳离子）的影响，为其临床应用提供科学依据。传染性喉部炎病毒（ILTV）共挑战了200鸡肉。在挑战后72小时，用不同剂量的YM或中草药侯燕京粉治疗鸡。检测ILTV的相对表达，气管的病理变化和SIGA分泌细胞的数量。薄层色谱结果证实，YM含有瘢痕疙瘩，Flos Lonicerae，Pericarpium Citri reticilatae和甘草。通过人工感染成功建立了阳离子模型。在高剂量YM组（HD）和中剂量YM组（MD）中，效果的有效率分别为100和96.7％，整体固化率为83.3％。此外，尸检结果表明鸡气管中的组织损伤程度相对较低。与阳性对照组相比，HD和MD鸡具有ILTV的相对表达，但更多的SIGA分泌细胞。总之，YM可以减少组织中的ILTV水平，减轻感染引起的组织损伤，增强对阳离子具有明显治疗作用的粘膜免疫力。

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