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首页> 外文期刊>Platelets >Do methodological differences account for the current controversy on tissue factor expression in platelets?
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Do methodological differences account for the current controversy on tissue factor expression in platelets?

机译：方法差异是否考虑了目前血小板组织因子表达的争议？

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摘要

Tissue factor (TF), the key activator of the blood coagulation cascade and of thrombus formation, is also expressed by circulating human platelets. Despite the documented in-depth characterization of platelet TF carried out in the past 15 years, some authors still fail to identify TF in platelets, especially when assessment in platelet-rich plasma (PRP) or washed platelets is carried out. This study aims to extend the characterization of the subset of TF-positive platelets in PRP from healthy subjects and to verify how different centrifugation forces, used to prepare the PRP, could affect the analysis of TF-positive platelets. Data indicate that large-size platelets express significantly higher amount of TF compared to small-size cells, in terms of both TF protein and TF mRNA. Upon stimulation, large platelets readily expose on the cell membrane TF, which is functionally active, i.e., able to generate factor Xa (FXa) as well as thrombin. By contrast, TF activity in small platelets is almost completely quenched by tissue factor pathway inhibitor (TFPI), becoming indeed detectable only after treatment with an anti-TFPI antibody.
机译：通过循环人血小板，还表达了组织因子（TF），血液凝固级联和血栓形成的关键活化剂。尽管过去15年进行了血小板TF的文献深入表征，但一些作者仍未识别血小板中的TF，特别是当进行富含血小板血浆（PRP）或洗涤的血小板中的评估时。该研究旨在扩展来自健康受试者的PRP中TF阳性血小板的子集的表征，并验证用于制备PRP的离心力的不同程度可能影响TF阳性血小板的分析。数据表明，对于TF蛋白和TF mRNA而言，大尺寸的血小板与小尺寸细胞相比明显较高的TF量。在刺激后，大血小板容易暴露在细胞膜TF上，其在功能上活性，即能够产生因子Xa（FXA）以及凝血酶。相比之下，小血小板中的TF活性几乎完全通过组织因子途径抑制剂（TFPI）淬灭，仅在用抗TFPI抗体处理后才能才能检测到。

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