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首页> 外文期刊>Progress in Artificial Intelligence >Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells
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Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells

机译：Pholiota NamekO多糖促进细胞增殖和迁移，并减少了H2O2诱导的L929细胞中的ROS含量

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摘要

Pholiota nameko, a type of edible and medicinal fungus, is currently grown extensively for food and traditional medicine in China and Japan. It possesses various biological activities, such as anti-inflammatory, anti-hyperlipidemia and antitumor activities. However, P. nameko has rarely been discussed in the field of dermatology; identifying its biological activities could be beneficial in development of a new natural ingredient used in wound care. To evaluate its in vitro wound healing activities, the present study assessed the antioxidant and anti-collagenase activities of P. nameko polysaccharides (PNPs) prepared through fractional precipitation (40%, 60% and 80% (v/v)); the assessments were conducted using reducing power, hydroxyl radical scavenging activity, dichloro-dihydro-fluorescein diacetate and collagenase activity assays. The ability of PNPs to facilitate L929 fibroblast cell proliferation and migration was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and scratch assays. The findings indicated that, among all fractions, PNP-80 showed the best antioxidant and anti-collagenase activity, as measured by their reducing power (IC50 of PNP-80 was 2.43 +/- 0.17 mg/mL), the hydroxyl radical scavenging (IC50 of PNP-80 was 2.74 +/- 0.11 mg/mL) and collagenase activity assay, and significantly reduced cellular ROS content, compared with that of H2O2-induced L929 cells. Moreover, PNP-80 significantly promoted L929 fibroblast proliferation and migration, compared with the control group. Overall, we suggested that PNP-80 could be a promising candidate for further evaluation of its potential application on wound healing.
机译：Pholiota Nameko，一种可食用和药用真菌，目前正在中国和日本广泛种植食品和传统医学。它具有各种生物活性，如抗炎，抗高脂血症和抗肿瘤活动。然而，P. Nameko很少在皮肤科领域讨论;确定其生物学活动可能是有益的，在伤口护理中的一种新的天然成分的发展中有益。为了评估其体外伤口愈合活性，本研究评估了通过分馏沉淀（40％，60％和80％（v / v）制备的P. nameko多糖（PNP）的抗氧化剂和抗胶原酶活性;使用还原功率，羟基自由基清除活性，二氯二氢 - 荧光素二乙酸酯和胶原酶活性测定进行评估。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴铵（MTT）和划痕测定评估PNP促进L929成纤维细胞增殖和迁移的能力。结果表明，在所有级分中，PNP-80显示最佳的抗氧化剂和抗胶原酶活性，通过它们的还原功率（PNP-80的IC50为2.43 +/- 0.17mg / ml），羟基自由基清除（与H2O2诱导的L929细胞相比，PNP-80的IC50为2.74 +/- 0.11mg / ml）和胶原酶活性测定，并显着降低细胞ROS含量。此外，与对照组相比，PNP-80显着促进了L929成纤维细胞增殖和迁移。总体而言，我们建议PNP-80可以成为进一步评估其对伤口愈合潜在申请的有希望的候选者。

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