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首页> 外文期刊>Progress in Artificial Intelligence >Insulin negatively regulates dedifferentiation of mouse adipocytes in vitro
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Insulin negatively regulates dedifferentiation of mouse adipocytes in vitro

机译：胰岛素对小鼠脂肪细胞的胰岛素产生负面影响

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摘要

Insulin plays an important role during adipogenic differentiation of animal preadipocytes and the maintenance of mature phenotypes. However, its role and mechanism in dedifferentiation of adipocyte remains unclear. This study investigated the effects of insulin on dedifferentiation of mice adipocytes, and the potential mechanisms. The preadipocytes were isolated from the subcutaneous white adipose tissue of wild type (WT), TNF alpha gene mutant (TNF alpha-/-), leptin gene spontaneous point mutant (db/db) and TNF alpha-/-/db/db mice and were then induced for differentiation. Interestingly, dedifferentiation of these adipocytes occurred once removing exogenous insulin from the adipogenic medium. As characteristics of dedifferentiation of the adipocytes, downregulation of adipogenic markers, upregulation of stemness markers and loss of intracellular lipids were observed from the four genotypes. Notably, dedifferentiation was occurring earlier if the insulin signal was blocked. These dedifferentiated cells regained the potentials of the stem cell-like characteristics. There is no significant difference in the characteristics of the dedifferentiation between the adipocytes. Overall, the study provided evidence that insulin plays a negative regulatory role in the dedifferentiation of adipocytes. We also confirmed that both dedifferentiation of mouse adipocytes, and effect of the insulin on this process were independent of the cell genotypes, while it is a widespread phenomenon in the adipocytes.
机译：胰岛素在动物前脂肪细胞的脂肪生成分化和维持成熟表型中起着重要作用。然而，它在脂肪细胞的去细胞中的作用和机制仍然尚不清楚。本研究研究了胰岛素对小鼠脂肪细胞的消化剂和潜在机制的影响。从野生型（WT），TNFα基因突变体（TNFα - / - ），瘦素基因自发点突变体（DB / DB）和TNFα - / - / dB / dB小鼠的皮下白色脂肪组织中分离了前脂肪细胞然后被诱导分化。有趣的是，在从脂肪介质中除去外源性胰岛素，这些脂肪细胞的去脱脂剂发生了去细胞。作为脂肪细胞的去细胞的特征，从四种基因型中观察到脂肪性标记的下调，患症状标记的上调和细胞内脂质的丧失。值得注意的是，如果胰岛素信号被阻断，早先发生消除剂。这些去生细胞重新恢复了干细胞状特征的电位。脂肪细胞之间消化不异的特征没有显着差异。总体而言，该研究提供了证据表明胰岛素在脂肪细胞的去脱脂剂中发挥了负调节作用。我们还证实，小鼠脂肪细胞的消化术和胰岛素对该过程的影响均与细胞基因型无关，而在脂肪细胞中是一种广泛的现象。

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