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New insights into the heterogeneous ripening in Hass avocado via LC-MS/MS proteomics

机译：通过LC-MS / MS蛋白质组学研究HASS鳄梨的异构成熟的新见解

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摘要

Hass avocado postharvest ripening heterogeneity creates several logistics problems at destination markets. Although several approaches mainly based on targeted analysis have been undertaken to broaden our understanding of the potential causes of this postharvest ripening heterogeneity, still the secret is not yet unveiled. In this study biopsies from individual fruit displaying contrasting ripening behavior (slow vs fast) and subjected to different storage conditions (immediately after harvest, 30 d regular air at 5 degrees C and 30 d at 5 degrees C and 4 kPa O-2 and 6 kPa CO2) were analyzed for targeted fatty acid analysis and cell wall enzyme activity combined with a more integrative approach based on gel free proteomics LC MS/MS. The high throughput proteomics was capable of discriminating between slow and fast ripening avocado fruit. Proteins that were mainly less abundant in the slow ripening phenotype were annotated to correct protein folding, translation and de novo synthesis and higher abundant proteins were annotated to amino sugar and nucleotide metabolism, detoxification and stress response and proteolysis. Our study opens new insights into the causes of heterogeneous ripening and may provide new markers for avocado ripening at harvest and after prolonged storage.
机译：Hass Avocado Postharvest成熟异质性在目的地市场创造了几个物流问题。虽然已经采取了几种主要基于有针对性分析的方法，但却拓宽了对这种采后成熟异质性的潜在原因的理解，但仍然尚未揭幕。在这项研究中，各个果实的活组织检查显示对比的成熟行为（快速慢）并经受不同的储存条件（在收获后立即，在5℃和30 d处在5℃和4kPa O-2和6的30d常规空气中进行30 d常规空气分析KPA CO2）用于靶向脂肪酸分析和细胞壁酶活性，基于凝胶游离蛋白质组学LCS MS / MS的更综合的方法。高通量蛋白质组学能够区分缓慢而快速成熟的鳄梨果实。主要在慢熟化表型中较小较小的蛋白质被注释为纠正蛋白质折叠，翻译和DE Novo合成和更高的丰富蛋白质被注释给氨基糖和核苷酸代谢，排毒和应激反应和蛋白水解。我们的研究开辟了异构成熟的原因的新见解，可以为收获和延长储存后的鳄梨成熟的新标志物。

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