Loop-Mediated Isothermal Amplification for Detection of the 5.8S Ribosomal Ribonucleic Acid Internal Transcribed Spacer 2 Gene Found in Trypanosoma brucei gambiense

Olga V. Nikolskaia; Oriel M. M. Thekisoe; J. Stephen Dumler; Dennis J. Grab

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Loop-Mediated Isothermal Amplification for Detection of the 5.8S Ribosomal Ribonucleic Acid Internal Transcribed Spacer 2 Gene Found in Trypanosoma brucei gambiense

机译：环介导的等温扩增用于检测5.8S核糖体核糖核核酸内转录的间隔2基因，在锥锥瘤中发现Brucei Gambiense

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The loop-mediated isothermal amplification (LAMP) assay with its advantages of cost effectiveness, rapidity, and simplicity, has evolved as a sensitive and specific method for the detection of African trypanosomes. Highly sensitive LAMP reactions specific for Trypanosoma brucei rhodesiense or that recognize but do not discriminate between Trypanosoma brucei brucei, T. b. rhodesiense, Trypanosoma brucei gambiense, and Trypanosoma evansi have been developed. A sensitive LAMP assay targeting the T. b. gambiense 5.8S ribosomal RNA internal transcribed spacer 2 (5.8S-ITS2) gene is also available but this assay does not target binding sites that span the CCCA (C3A) (557-560 bps) insertion site that further differentiates T. b. gambiense from T. b. brucei. Here we describe 5.8S-ITS2-targeted LAMP assay that fit these criteria. The LAMP primer sets containing the T. b. gambiense-specific C3A tetranucleotide at the start of the outer forward primer sequences showed high specificity and sensitivity down to at least 0.1 fg T. b. gambiense genomic DNA.

机译：环介导的等温扩增（灯）测定具有成本效益，快速性和简单性的优点，它已经发展为检测非洲锥虫的敏感和具体方法。对锥虫瘤的高度敏感的灯反应，对葡萄球菌瘤Brucei rhodesiense或识别但不区分锥形瘤Brucei Brucei，T.B。已经开发出rhodeSiense，替妥塞诺氏蛋白瘤Brucei Gambiense和雷帕诺血瘤埃瓦西。靶向T.B的敏感灯测定。 Gambiense 5.8S核糖体RNA内部转录的间隔件2（5.8S-ITS2）基因也可用，但该测定不瞄准跨越CCCA（C3A）（557-560bps）插入位点的结合位点，其进一步区分T.b。来自T.B的甘比塞。布鲁迪。在这里，我们描述了适合这些标准的5.8S-ITS2目标灯测定。含有T.b的灯引物组。外部前后引物序列开始时的甘甜特异性C3a四核苷酸显示出高的特异性和敏感性下降至至少0.1fg t.b。 Gambiense基因组DNA。

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《The American Journal of Tropical Medicine and Hygiene》 |2017年第2期|共5页
作者
Olga V. Nikolskaia; Oriel M. M. Thekisoe; J. Stephen Dumler; Dennis J. Grab;
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Department of Pathology The Johns Hopkins University School of Medicine Baltimore Maryland;

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正文语种 eng
中图分类地方病学;
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1. Loop-Mediated Isothermal Amplification for Detection of the 5.8S Ribosomal Ribonucleic Acid Internal Transcribed Spacer 2 Gene Found in Trypanosoma brucei gambiense [J] . Olga V. Nikolskaia, Oriel M. M. Thekisoe, J. Stephen Dumler, The American Journal of Tropical Medicine and Hygiene . 2017,第2期

机译：环介导的等温扩增用于检测5.8S核糖体核糖核核酸内转录的间隔2基因，在锥锥瘤中发现Brucei Gambiense
2. Loop-mediated Isothermal Amplification and nested PCR of the Internal Transcribed Spacer (ITS) for Histoplasma capsulatum detection [J] . Matheus da Silva Zatti, Thales Domingos Arantes, José Alex Louren?o Fernandes, PLOS Neglected Tropical Diseases . 2019,第8期

机译：环状转录等温扩增和内部转录间隔物（ITS）的巢式PCR用于组织胞浆囊肿的检测
3. Detection of Kudoa Septempunctata in the Japanese Flounder by Loop-Mediated Isothermal Amplification (LAMP) Targeting the Internal Transcribed Spacer (ITS) Regions [J] . Gouranga Biswas, Hirono Kodama, Tohru Mekata, Turkish Journal of Fisheries & Aquatic Sciences . 2016,第3期

机译：通过靶向内部转录间隔区（ITS）区域的环介导的等温扩增（LAMP）检测日本比目鱼中的Kudoa Septempunctata。
4. Amplifying and Sequencing Analysis the Internal Transcribed Spacer (ITS) Regions of Olpidium Viciae Kusano's Ribosomal DNA in Broad Bean [C] . Jiming Yan, Xiaohong Shi, Miao Mei, International Conference on Advanced Materials and Information Technology Processing . 2011

机译：放大和测序分析植物中奥匹吡氏菌的内部转录间隔物（ITS）区域在蚕豆中的核糖体DNA
5. Phylogeny and biogeography of Gnetum (Gnetales): I. Evolution of the internal transcribed spacer of nuclear ribosomal DNA in a genus of gymnosperms, Gnetum; II. Molecular evolution of the chloroplast trnT-trnF region: Insights from Gnetales; III. Horizontal gene transfer from flowering plants to Gnetum (Gnetales); and, IV. Age, evolution, and biogeography of Gnetum [D] . Won, Hyosig 2004

机译：Gnetum（Gnetales）的系统发育和生物地理学：I.裸子植物Gnetum核内核糖体DNA内部转录间隔区的演变；二。叶绿体trnT-trnF区的分子进化：来自Gnetales的见解；三，基因水平从开花植物转移到Gnetum（Gnetales）；并且，IV。 Gnetum的年龄，进化和生物地理
6. Loop-Mediated Isothermal Amplification for Detection of the 5.8S Ribosomal Ribonucleic Acid Internal Transcribed Spacer 2 Gene Found in Trypanosoma brucei gambiense [O] . Olga V. Nikolskaia, Oriel M. M. Thekisoe, J. Stephen Dumler, 2017

机译：环介导的等温扩增用于检测布氏布鲁氏菌中发现的5.8S核糖体核糖核酸内部转录间隔区2基因
7. Detection of group 1 Trypanosoma brucei gambiense by loop-mediated isothermal amplification [O] . Njiru Z. K., Traub R. J., Ouma J. O., 2011

机译：环介导的等温扩增法检测布鲁氏甘蓝氏锥虫1组

Loop-Mediated Isothermal Amplification for Detection of the 5.8S Ribosomal Ribonucleic Acid Internal Transcribed Spacer 2 Gene Found in Trypanosoma brucei gambiense

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