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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Effect of Gamma Irradiation on the Antibody Response Measured in Human Serum from Subjects Vaccinated with Recombinant Vesicular Stomatitis Virus-Zaire Ebola Virus Envelope Glycoprotein Vaccine
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Effect of Gamma Irradiation on the Antibody Response Measured in Human Serum from Subjects Vaccinated with Recombinant Vesicular Stomatitis Virus-Zaire Ebola Virus Envelope Glycoprotein Vaccine

机译：γ辐射对来自重组囊泡炎病毒封闭术血栓母蛋白血糖蛋白疫苗疫苗疫苗受试者中人血清中测量的抗体反应的影响

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摘要

rVSV Delta G-ZEBOV-GP vaccine is a live recombinant (r) vesicular stomatitis virus (VSV), where the VSV G protein is replaced with the Zaire Ebola virus (ZEBOV) glycoprotein (GP). For vaccine immunogenicity testing, clinical trial sera collected during an active ZEBOV outbreak underwent gamma irradiation (GI) before testing in biosafety level 2 laboratories to inactivate possible wild-type ZEBOV. Before irradiating pivotal trial samples, two independent studies evaluated the impact of GI (50 kGy) on binding ZEBOV-GP (ELISA) antibodies against rVSV Delta G-ZEBOV-GP, using sera from a North American phase 1 study. Gamma irradiation was associated with slightly higher antibody concentrations in pre-vaccination samples and slightly lower concentrations postvaccination. Results indicate that GI is a viable method for treating samples from regions where filoviruses are endemic, with minor effects on antibody titers. The impact of GI on immunogenicity analyses should be considered when interpreting data from irradiated specimens.
机译：RVSV Delta G-Zebov-GP疫苗是一种活重组（R）叠囊炎病毒（VSV），其中VSV G蛋白被Zaire Ebola病毒（Zebov）糖蛋白（GP）取代。对于疫苗免疫原性测试，在生物安全2级实验室中测试前γ辐射（GI）的活性Zebov爆发期间收集的临床试验血清，以灭活可能的野生型Zebov。在照射枢轴试验样品之前，使用来自北美第1研究的血清，两种独立研究评估了GI（50kGy）对针对RVSV Delta G-Zebov-GP的结合Zebov-GP（ELISA）抗体的影响。 γ辐射与预接种疫苗样品中的较高抗体浓度有关，略低较低的后效率。结果表明，GI是一种可行的方法，用于处理来自血管病毒的区域是流动的，对抗体滴度有微小的影响。在解释来自辐照标本的数据时，应考虑GI对免疫原性分析的影响。

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