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首页> 外文期刊>The Australasian journal of dermatology >Protective effect of skin-derived precursors on photoaging in nude mice
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Protective effect of skin-derived precursors on photoaging in nude mice

机译：皮肤源性前体在裸鼠中的光学手册中的保护作用

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Objectives Currently, innovative methods to prevent photoaging are needed. Skin-derived precursors (SKP) have been shown to play a crucial role in resisting UVB-induced apoptosis in vitro. The objective of this study was to explore the effect of SKP on preventing skin photoaging in vivo. Methods Skin-derived precursors from neonatal BALB/c mice were isolated, identified and intradermally transplanted with a PKH26 label to track their survival. These were then injected at different concentrations into the buttock dermis of nude mice at 2-weekly intervals before UV irradiation. Photographs, assessment of live skin surface, histology with quantitative real-time polymerase chain reaction and immunohistochemistry were used to evaluate the impact of SKP on wrinkles and other relevant indicators of skin photoaging. Results SKP exhibited a sphere-like structure and could survive for at least 2 weeks after intradermal transplantation. A large dose of SKP transplantation (10(5) SKP +UV) at 2-weekly intervals were able to ameliorate coarse UV-induced wrinkles. Moreover, the skin smoothness value, dermal thickness and collagen percentage were significantly increased in mice that received a large dose of SKP (10(5) SKP +UV). UV radiation induced the mRNA expression of MMP-13 and decreased the mRNA and protein expression of T beta RII, but these effects were diminished by SKP transplantation. The transplantation of SKP could increase the mRNA of TIMP-1. Conclusions We found that transplanted SKP exert a beneficial impact on preventing UV-induced wrinkles in vivo, suggesting that SKP transplantation is a promising candidate for preventing photoaging.
机译：目前目前，需要采用创新方法来防止使用光电。已显示皮肤衍生的前体（SKP）在体外抵抗UVB诱导的细胞凋亡中起着至关重要的作用。本研究的目的是探讨SKP在体内防止皮肤光学的影响。方法用PKH26标记分离出新生儿BALB / C小鼠的皮肤衍生的前体，鉴定和皮内移植，以跟踪它们的存活。然后将这些以不同浓度的浓度注入裸鼠的臀部真菌，在紫外线照射前为2-每周间隔。摄影，活皮肤表面的评估，具有定量实时聚合酶链反应和免疫组化的组织学用于评估SKP对皮肤皱纹和其他相关指标的影响。结果SKP展现出球形结构，在皮内移植后至少2周内存活。大量的SKP移植（10（5）个SKP + UV）以2-每周间隔才能改善粗紫外线诱导的皱纹。此外，在接受大剂量的SKP（10（5）SKP + UV）的小鼠中，皮肤光滑度值，皮肤厚度和胶原百分比显着增加。紫外线辐射诱导MMP-13的mRNA表达并降低了TβRII的mRNA和蛋白表达，但通过SKP移植减少了这些效应。 SKP的移植可以增加TIMP-1的mRNA。结论我们发现移植的SKP对预防紫外线诱导的体内皱纹产生有益的影响，表明SKP移植是预防照片的有希望的候选者。

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