...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Chronic pharmacological blockade of the Na+/Ca2+ exchanger modulates the growth and development of the Purkinje cell dendritic arbor in mouse cerebellar slice cultures
【24h】

Chronic pharmacological blockade of the Na+/Ca2+ exchanger modulates the growth and development of the Purkinje cell dendritic arbor in mouse cerebellar slice cultures

机译：Na + / Ca2 +交换剂的慢性药理学阻断调节小鼠小脑切片培养物中Purkinje细胞树突树突的生长和发育

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Na+/Ca2+ exchanger (NCX) is a bidirectional plasma membrane antiporter involved in Ca2+ homeostasis in eukaryotes. NCX has three isoforms, NCX1-3, and all of them are expressed in the cerebellum. Immunostaining on cerebellar slice cultures indicates that NCX is widely expressed in the cerebellum, including expression in Purkinje cells. The pharmacological blockade of the forward mode of NCX (Ca2+ efflux mode) by bepridil moderately inhibited growth and development of Purkinje cell dendritic arbor in cerebellar slice cultures. However, the blockade of the reverse mode (Ca2+ influx mode) by KB-R7943 severely reduced the dendritic arbor and induced a morphological change with thickened distal dendrites. The effect of KB-R7943 on dendritic growth was unrelated to the activity of voltage-gated calcium channels and was also apparent in the absence of bioelectrical activity indicating that it was mediated by NCX expressed in Purkinje cells. We have used additional NCX inhibitors including CB-DMB, ORM-10103, SEA0400, YM-244769, and SN-6 which have higher specificity for NCX isoforms and target either the forward, reverse, or both modes. These inhibitors caused a strong dendritic reduction similar to that seen with KB-R7943, but did not elicit thickening of distal dendrites. Our findings indicate that disturbance of the NCX-dependent calcium transport in Purkinje cells induces a reduction of dendritic arbor, which is presumably caused by changes in the calcium handling, and underline the importance of the calcium equilibrium for the dendritic development in cerebellar Purkinje cells.
机译：Na + / Ca2 +交换器（NCX）是一种双向血浆膜抗膜，其参与真核生物中的CA2 +稳态。 NCX有三种同种型，NCX1-3，所有的全部在小脑中表达。小脑切片培养物上的免疫染色表明NCX在小脑中广泛表达，包括在PurkinJe细胞中的表达。 Bepridil的NCX（Ca2 +流出模式）的前向模式的药理阻滞中等抑制小脑切片培养物中紫癜细胞树突骨库的生长和发育。然而，通过KB-R7943的反向模式（CA2 +流入模式）的阻断严重降低了树突树枝状树叶，并诱导了与增厚的远端树枝状曲线的形态变化。 KB-R7943对树枝状生长的影响与电压门控钙通道的活性无关，并且在没有生物电活性的情况下也显而易见，这表明它是在紫癜细胞中表达的NCL介导的。我们使用了额外的NCX抑制剂，包括CB-DMB，ORM-10103，SEA0400，YM-244769和SN-6，其对NCX同种型具有更高的特异性，并且靶向前进，反向或两种模式。这些抑制剂引起了与KB-R7943相似的强烈树突还原，但未引发远端树枝状的增厚。我们的研究结果表明，Purkinje细胞中NCX依赖性钙转运的干扰诱导树突乔木的减少，这可能是由钙处理的变化引起的，并强调了小脑紫癜细胞中树突发育的钙均衡的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号