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首页> 外文期刊>The European Journal of Neuroscience >Arginine vasopressin differentially modulates GABAergic synaptic transmission onto temperature-sensitive and temperature-insensitive neurons in the rat preoptic area
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Arginine vasopressin differentially modulates GABAergic synaptic transmission onto temperature-sensitive and temperature-insensitive neurons in the rat preoptic area

机译：精氨酸血管加压素差异地调节大鼠偏光区域的温度敏感和温度不敏感神经元的胃肠杆菌突触传递

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摘要

The preoptic area (POA) of the hypothalamus, containing temperature-sensitive and temperature-insensitive neurons, plays a key role in specific thermoregulatory responses. Although arginine vasopressin (AVP) has been shown to induce hypothermia by increasing the firing activities of warm-sensitive neurons and decreasing those of cold-sensitive and temperature-insensitive neurons, the effects of AVP on POA GABAergic transmission remain unknown. Herein, inhibitory postsynaptic currents (IPSCs) of temperature-sensitive and temperature-insensitive neurons in POA slices were recorded using whole-cell patch clamp. By monitoring changes in GABAergic transmission during AVP treatment, we showed that AVP decreased the amplitudes and frequencies of spontaneous IPSCs in mostly warm-sensitive neurons and in some temperature-insensitive neurons but increased these parameters in other temperature-insensitive neurons. The IPSC amplitude was reduced for only cold-sensitive neurons. RT-PCR and Western blot analyses further confirmed the POA expression of V1a receptors and GABAA receptors, including the subunits alpha1, alpha2, alpha3, ebta2, beta3 and gamma2. The effects of AVP on IPSCs in temperature-sensitive and temperature-insensitive neurons were dependent on G proteins and intracellular Ca2+. AVP-mediated modulation was associated with changes in the kinetic parameters (decay time, 10-90% rise time, half-width). Together, these results suggest that AVP, acting via Via receptors but not V1b receptors, differentially modulates GABAergic synaptic transmission and fine-tunes the firing activities of temperature-sensitive and temperature-insensitive neurons in the rat POA.
机译：含有温度敏感和温度不敏感神经元的下丘脑的突出区域（POA）在特定的热调节反应中起着关键作用。虽然已经显示精氨酸血管加压素（AVP）通过增加温敏感神经元的烧制活性和降低冷敏感和温度不敏感神经元的烧制活性，但AVP对POA Gabaeric传输的影响仍然未知。这里，使用全细胞贴片夹子记录POA切片中温度敏感和温度不敏感神经元的抑制突触突触（IPSC）。通过监测AVP治疗期间GABA能传输的变化，我们表明AVP在大多数温敏神经元和一些温度不敏感神经元中减少了自发性IPSC的幅度和频率，但在其他温度不敏感神经元中增加了这些参数。仅降低IPSC振幅仅为冷敏感性神经元。 RT-PCR和Western印迹分析进一步证实了V1A受体和GABAA受体的POA表达，包括亚基α1，α2，α3，EBTA2，β3和γ2。 AVP对温度敏感和温度不敏感神经元IPSC的影响取决于G蛋白和细胞内Ca2 +。 AVP介导的调节与动力学参数的变化有关（衰减时间，10-90％上升时间，半宽）。这些结果表明，AVP通过受体而不是V1B受体，差异地调节胃肠杆菌突触传递和微调大鼠POA中温度敏感和温度不敏感神经元的烧制活性。

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