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首页> 外文期刊>The international journal of developmental biology >Keeping amphioxus in the laboratory: an update on available husbandry methods
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Keeping amphioxus in the laboratory: an update on available husbandry methods

机译：在实验室中保持Amphioxus：有关可用畜牧方法的更新

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Cephalochordates, commonly known as amphioxus or lancelets, are small, marine animals that can be found in coastal habitats of temperate, subtropical, and tropical waters. Together with vertebrates and tunicates, the cephalochordates belong to the chordate phylum, whose members are characterized by a number of conserved morphological features, such as a dorsal nerve cord, a notochord, a pharynx, a segmented musculature as well as a post-anal tail. Due to their basal position within the phylum, cephalochordates have become essential models for studying the evolutionary origin and diversification of vertebrates. Here, we present the currently available methods for maintaining and rearing cephalochordates in a laboratory environment, focusing on five species: the European amphioxus (Branchiostoma lanceolatum), the Florida amphioxus (Branchiostoma floridae), the Chinese amphioxus (Branchiostoma belcheri), the Japanese amphioxus (Branchiostoma japonicum), and the Bahamas lancelet (Asymmetron lucayanum). In addition to reviewing the protocols for capture, transport, aquaculture, and feeding of adults, we discuss methods for controlling gonad development and spawning, as well as for growing embryos and larvae. This information is complemented by observations from our animal facility on the European amphioxus (Branchiostoma lanceolatum). In sum, this work summarizes the latest advances in cephalochordate animal husbandry and highlights challenges for improving the use of these animals as laboratory model systems.
机译：Cephalochordates，俗称Amphioxus或Lancelets，是小型的海洋动物，可以在温带，亚热带和热带水域的沿海栖息地找到。与脊椎动物和姿势一起，头孢菌素属于脊髓字形，其成员的特征在于许多保守的形态特征，例如背神经帘线，脊索，咽部，分段的肌肉组织以及肛门后尾巴。由于它们在门区内的基础位置，Cephalochates已经成为研究进化源和脊椎动物多样化的基本模型。在这里，我们介绍了在实验室环境中维护和饲养头部的现有方法，专注于五种物种：欧洲Amphioxus（Branchiostoma Lanceolatum），佛罗里达疗法（Brankiostoma Floridae），中国疗法（Branchiostoma Belcheri），日本Amphioxus （日本肺腹瘤），以及巴哈马州兰开德（Asymmetron Lucadanum）。除了审查成人捕获，运输，水产养殖和饲养方面的议定书，我们讨论控制Gonad开发和产卵的方法，以及种植胚胎和幼虫。这些信息由我们在欧洲Amphioxus（Branchiostoma Lanceolatum）的动物设施的观察结果补充。总而言之，这项工作总结了Cephalochordate畜牧业的最新进展，并突出了改善这些动物作为实验室模型系统的挑战。

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