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首页> 外文期刊>The Journal of Agricultural Science >Development of interspecific hybrids of cassava and paternity analysis with molecular markers.
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Development of interspecific hybrids of cassava and paternity analysis with molecular markers.

机译：分子标志物的木薯和亲子分析的发展杂交。

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摘要

The present paper demonstrates the development of interspecific hybrids between Manihot esculenta Crantz ssp. esculenta (Mee) and M. esculenta Crantz ssp. flabellifolia (Mef) and paternity analysis using microsatellite markers [simple sequence repeat (SSR)]. Three Mef accessions (FLA005, FLA025V and FLA029V) were used for crosses with varieties of Mee: Saracura, Aipim Bravo, COL 1725, Aipim Rosa, Abobora, Parana and PER334. The paternity of the interspecific hybrids was investigated using 24 SSRs. The observed heterozygosity (Ho), polymorphic information content (PIC), probability of identity (PI) and paternity exclusion (PE) were evaluated. The rate of breeding success varied from 17 to 92%, and an average of two pollinations were required for each generated hybrid plant. The Ho value ranged from 0.11 to 0.92, and the PIC value ranged from 0.12 to 0.59. The uneven distribution of allele frequencies was accompanied by a high PI average (0.56). However, the combined PE for 21 loci was 0.99, which allows for the determination of the paternity of the hybrids with good discriminatory power. Of the 74 hybrids evaluated, 0.82 had their paternity confirmed using microsatellite markers. Discriminant analysis of principal components (DAPC) indicated the presence of eight clusters, of which, one was composed of only Mef varieties and the supposed hybrid Fla52Sar-H7, which was a product of apomixis. The parent Mee and hybrids were allocated in the other seven clusters. The data obtained demonstrate that SSR markers can be routinely used in breeding programmes to verify the paternity of interspecific crosses of cassava.
机译：本文展示了Manihot Esculenta Crantz SSP之间的三种杂种的发展。 Ecculenta（Mee）和M. Esculenta Crantz SSP。使用微卫星标记的Flabellifolia（MEF）和亲子鉴率分析[简单序列重复（SSR）]。三个MeF附加（FLA005，FLA025V和FLA029V）用于与各种Mee的十字架：Saracura，AIPIM Bravo，Col 1725，Aipim Rosa，Abobora，Parana和Per334。使用24个SSRS研究了三种杂交种的亲态性。观察到的杂合性（HO），多态信息含量（PIC），同一性（PI）和亲子排除（PE）的概率。育种成功率从17升至92％，每种生成的杂化厂都需要平均两种授粉。 HO值范围为0.11至0.92，PIC值范围为0.12至0.59。等位基因频率的不均匀分布伴有高pi平均值（0.56）。然而，21个基因座的合并PE为0.99，其允许用良好的歧视性测定杂种的亲子率。在评估的74个杂交种中，0.82具有它们的亲子关系，使用微卫星标记证实。主成分（DAPC）的判别分析表明存在八种簇，其中一个是仅由MEF品种和假定的杂交FLA52SAR-H7组成，这是apomixis的产物。在其他七个集群中分配了父级和混合动力车。所获得的数据表明，SSR标记可以常规用于育种程序以验证木薯的间隙杂交的亲子关系。

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