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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >In situ assessment of mitochondrial calcium transport in tobacco pollen tubes
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In situ assessment of mitochondrial calcium transport in tobacco pollen tubes

机译:烟草花粉管中线粒体钙运输的原位评估

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摘要

Pollen tubes require functional mitochondria in order to achieve fast and sustained growth. In addition, cell wall expansion requires a calcium gradient in the tube apex formed by a dedicated array of calcium pumps and channels. Most studies have traditionally focused on the molecular aspects of calcium interactions and transport across the pollen tube plasmalemma. However, calcium transients across mitochondrial membranes from pollen tubes are beginning to be studied. Here, we report the presence of a ruthenium red-sensitive mitochondrial calcium uniporter-like activity in tobacco pollen tubes with functional oxidative phosphorylation. The present study provides a framework to measure in situ specifics of mitochondrial transport and respiration in pollen tubes from different plants. The relevance of a mitochondrial calcium uniporter for pollen tube growth is discussed.
机译:花粉管需要功能性线粒体,以实现快速和持续增长。 此外,细胞壁膨胀需要通过专用钙泵和通道形成的管顶点中的钙梯度。 传统上,大多数研究集中于钙相互作用的分子方面和整个花粉管等离子体的分子。 然而,开始研究来自花粉管的线粒体膜的钙瞬变。 在这里,我们在具有功能性氧化磷酸化的烟草花粉管中报告存在钌红敏感线粒体钙的单钙样活性。 本研究提供了一种框架,用于测量来自不同植物的花粉管中的线粒体输送和呼吸的原位细节。 讨论了线粒体钙二百六加仑对花粉管生长的相关性。

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