...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Virus-induced gene silencing in Rauwolfia species
【24h】

Virus-induced gene silencing in Rauwolfia species

机译：RAUWOLFIA物种的病毒诱导基因沉默

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Elucidation of the monoterpene indole alkaloid biosynthesis has recently progressed in Apocynaceae through the concomitant development of transcriptomic analyses and reverse genetic approaches performed by virus-induced gene silencing (VIGS). While most of these tools have been primarily adapted for the Madagascar periwinkle (Catharanthus roseus), the VIGS procedure has scarcely been used on other Apocynaceae species. For instance, Rauwolfia sp. constitutes a unique source of specific and valuable monoterpene indole alkaloids such as the hypertensive reserpine but are also well recognized models for studying alkaloid metabolism, and as such would benefit from an efficient VIGS procedure. By taking advantage of a recent modification in the inoculation method of the Tobacco rattle virus vectors via particle bombardment, we demonstrated that the biolistic-mediated VIGS approach can be readily used to silence genes in both Rauwolfia tetraphylla and Rauwolfia serpentina. After establishing the bombardment conditions minimizing injuries to the transformed plantlets, gene downregulation efficiency was evaluated at approximately a 70% expression decrease in both species by silencing the phytoene desaturase encoding gene. Such a gene silencing approach will thus constitute a critical tool to identify and characterize genes involved in alkaloid biosynthesis in both of these prominent Rauwolfia species.
机译：通过伴随的转录组分析和病毒诱导的基因沉默（Vigs）进行的转录组分析和逆向遗传方法的伴随发育，最近在Apocynaceae中进行了单萜吲哚生物碱生物合成的阐明。虽然大多数这些工具主要适用于Madagascar Periwinkle（Catharanthus Roseus），但Vigs手术几乎没有用于其他Apocynaceae物种。例如，Rauwolfia sp。构成特异性和有价值的单萜吲哚生物碱如高血压沉重腺嘌呤的独特来源，但也是研究生物碱代谢的公认模型，因此将受益于有效的Vigs程序。通过利用粒子轰击的烟草拨浪鼓病毒载体的接种方法最近的修改，我们证明了生物介导的Vigs方法可以容易地用于Rauwolfia Tetraphylla和Rauwolfia Serpentina的沉默基因。在建立轰击条件最小化转化的植物损伤后，通过沉默植物去饱和酶编码基因在两种物种中的约70％表达下评估基因下调效率。因此，这种基因沉默方法将构成识别和表征在这些突出的RAUWIA种类中涉及生物碱生物合成中所涉及的基因的关键工具。

著录项

  • 来源
  • 作者

    Corbin Cyrielle; Lafontaine Florent; Sepulveda Liuda Johana; Carqueijeiro Ines; Courtois Martine; Lanoue Arnaud; de Bernonville Thomas Duge; Besseau Sebastien; Glevarec Gaelle; Papon Nicolas; Atehortua Lucia; Giglioli-Guivarch Nathalie; Clastre Marc; St-Pierre Benoit; Oudin Audrey; Courdavault Vincent;

  • 作者单位

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Angers Grp Etud Interact Hote Pathogene EA 3142 Angers France;

    Univ Antioquia Sede Invest Univ Lab Biotecnol Antioquia Medellin Colombia;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞形态学;
  • 关键词

    Rauwolfia; Virus-induced gene silencing; Phytoene desaturase; Medicinal plant;

    机译：RAUWOLFIA;病毒诱导的基因沉默;植物去植物;药用植物;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号