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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Biochemical characterization and expression analysis of lignification in two pear (Pyrus ussuriensis Maxim.) varieties with contrasting stone cell content
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Biochemical characterization and expression analysis of lignification in two pear (Pyrus ussuriensis Maxim.) varieties with contrasting stone cell content

机译：两种梨（Pyrus Ussuriensis Maxim.）生物化学特征及表达分析，具有对比色细胞含量的品种

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摘要

As lignified stone cells reduce fruit quality, we investigated lignin deposition, phenolic metabolites, and expression of lignin biosynthetic genes during fruit development to elucidate the molecular mechanism of stone cell lignification using histological, biochemical, and transcriptional data from two Ussurian pear varieties (Jianba and Nanguo) with contrasting stone cell content. Lignin content and distribution coincided with stone cell accumulation. As per LC-MS analysis, Jianba exhibited higher levels of lignin monomers and hydroxycinnamates than Nanguo, consistently with lignin amount in each case. However, flavonoid content was much higher in Nanguo. Transcriptional data showed that most monolignol biosynthesis-related genes were particularly upregulated in Jianba during lignin accumulation; especially CCR and LAC, two monolignol biosynthesis-specific genes, were substantially upregulated in Jianba fruits at critical stages. Therefore, differences in stone cell content between "Jianba" and "Nanguo" may result from differential expression of lignin synthase genes located downstream of the lignin biosynthesis pathway. Taken together, our data may provide a deeper understanding of the molecular mechanism for stone cell lignification in pear fruit.
机译：作为荔枝的石细胞降低了水果质量，我们研究了木质素沉积，酚醛代谢物和木质素生物合成基因的表达在果实开发过程中，阐明了使用来自两个南苏里亚梨品种的组织学，生物化学和转录数据的石细胞瘫痪的分子机制（Jianba和nanguo）具有对比的石细胞含量。木质素含量和分布恰逢石材电池积累。根据LC-MS分析，Jianba始终表现出比Nanguo更高水平的木质素单体和羟基霉素，始终如一的木质素量。然而，Nanguo的黄酮含量高得多。转录数据表明，在木质素积累期间，在JianBA中，大多数单醇生物合成相关基因特别上调;特别是CCR和LAC，两种单醇生物合成特异性基因在临界阶段的JianBA果实中基本上上调。因此，“JianBA”和“Nanguo”之间的石细胞含量的差异可能是由位于木质素生物合成途径下游的木质素合酶基因的差异表达产生。我们的数据集合在一起，可以更深入地了解梨果中的石细胞瘫痪的分子机制。

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