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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Effect of the duration of use of aluminum cookware on its metal leachability and cytogenotoxicity in Allium cepa assay
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Effect of the duration of use of aluminum cookware on its metal leachability and cytogenotoxicity in Allium cepa assay

机译：铝炊具持续时间对CEPA测定中金属渗滤性和细胞诱导性的影响

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Aluminum cookware are widely used in many parts of the world. Data is increasing on the leaching of toxic metals from aluminum cookware into food and drink. In the present study, cytogenotoxicity of water boiled in three different aluminum pots (new, 3-year-old, and 6-year-old) in onion root tip's dividing cells was evaluated using theAllium cepaassay. The concentrations of Pb, As, Cd, Cu, Ni, and Al in the samples were also analyzed. Onion bulbs were grown in the boiled water samples, while tap water served as the control. Cytological and genetic analyses were carried out after 48 h, while analysis of inhibition of root length was carried out after 72 h. The results showed a significant (p < 0.05) cell proliferation and root growth inhibition compared with the control, which is dependent on the duration of use of the aluminum pots. The boiled water samples also caused modification of the root morphology as well as chromosomal aberrations which include sticky chromosomes, anaphase bridge, and disturbed spindle. The highest cytogenotoxicity was observed in the 6-year-old aluminum pot and the least in the new aluminum pot. Pb, As, Cd, Cu, Ni, and Al analyzed in the samples, with the highest concentrations in the 6-year-old aluminum pot, were believed to be responsible for the cytogenotoxicity observed in theA. cepaassay. The data of this study are indications that the aluminum pot-boiled water contains substances with the potential to be cytotoxic and cause mutations in somatic cells of A. cepa.
机译：铝制炊具广泛应用于世界的许多地方。数据正在增加铝炊具中毒金属的浸入食品和饮料。在本研究中，使用TheAllium Cepaassay评估三种不同铝罐（新，3岁和6岁）的水煮沸的水毒性毒素（新，3岁和6岁）。还分析了样品中的Pb，如Cd，Cu，Ni和Al的浓度。洋葱灯泡在煮沸的水样中生长，而自来水用作对照。在48小时后进行细胞学和遗传分析，同时在72小时后进行根长的抑制分析。结果表明，与对照相比，该结果表现出显着的（P <0.05）细胞增殖和根生长抑制，这取决于铝罐的使用持续时间。煮沸的水样也引起了根系形态的修饰以及包括粘性染色体，神木桥和干扰的主轴的染色体像差。在6岁的铝罐和新的铝罐中至少观察到最高细胞瘤毒性。在样品中分析的Pb，如CD，Cu，Ni和Al，据信，具有6岁铝罐中最高浓度的浓度，据信是在THEA中观察到的细胞诱导毒性的原因。 cepaassay。本研究的数据是铝制锅煮沸的水含有含有细胞毒性的物质并导致A.CEPA的体细胞突变的物质。

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