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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
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Technique for the identification of osmophores in flowers of herbarium material (TIOFH)

机译:植物标本料(TIOFH)花中渗透渗透压的技术

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摘要

The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96 degrees and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.
机译:搜索渗透杂肠的组织化学研究在其遵循的协议中不同于样品的固定和变色,以及所使用的污渍的类型和数量。 尽管存在这些差异,但所有的研究都有一个共同点:使用新鲜材料直接在田间收集或在植物园和温室中栽培。 这是对渗透学研究的显而易见的限制。 收集和不同植物家庭不同日期的植物标料标本的花卉部分暴露于变色剂的可变时间和百分比,并在不同的污渍下找到,以发现可以在这种类型的材料中积极测试渗透镜。 我们获得了用乙醇96℃和次氯酸钠的样品将样品变成了样品,并用Lugol,油红O和中性红色(TiOFH)染色。 提出了将这三个污渍结合成单个过程的渗透渗透测试的协议(TIOFH3)。

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