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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Virus-induced gene silencing (VIGS) for functional analysis of MYB80 gene involved in Solanum lycopersicum cold tolerance
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Virus-induced gene silencing (VIGS) for functional analysis of MYB80 gene involved in Solanum lycopersicum cold tolerance

机译：病毒诱导的基因沉默（Vigs）用于粘性菌菌凝固性凝固性茄子的MYB80基因的功能分析

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摘要

Virus-induced gene silencing (VIGS) is a method for transiently silencing genes in dicot and monocot plants. To evaluate the effects of chilling injury on activity of the MYB80 gene in Solanum lycopersicum, an investigation was conducted by VIGS using TRV-GFP (a modified TRV vector) to silence the target gene. During the chilling treatment (4/12 degrees C, 8/16-h dark/light photoperiod, with 60% humidity), the leaves were collected to analyze the malondialdehyde (MDA) content, soluble sugar content, free proline levels, and relative electric conductivity (REC). Leaves collected 2weeks after chilling treatment were used to detect the in situ accumulation of superoxide radical (O-2(-)). Additionally, we collected leaves at selected time points for semi-quantitative reverse transcription-PCR (RT-PCR) analysis. Eventually, 20days after chilling treatment, all plants were evaluated at 4 degrees C for 7days to assess the chilling injury index. The results validated that the MYB80 gene was related to cold tolerance of tomato plants, and that silencing of the MYB80 gene reduced the cold resistance ability.
机译：病毒诱导的基因沉默（Vigs）是Dicot和单子植物中瞬时沉默基因的方法。为了评估冷却损伤对溶兰氏菌菌菌菌菌菌菌菌菌菌菌菌菌菌菌菌的活性的影响，使用TRV-GFP（修饰的TRV载体）对静态靶基因进行静态进行调查。在冷却处理期间（4/12℃，8/16-H黑色/光照周周期，湿度为60％），收集叶片以分析丙二醛（MDA）含量，可溶性糖含量，游离脯氨酸水平和相对电导率（REC）。在冷却处理后，叶子收集了2周，用于检测超氧化物自由基的原位积累（O-2（ - ））。另外，我们在选定的时间点收集叶片，用于半定量逆转录-PCR（RT-PCR）分析。最终，20天后冷却治疗后，所有植物在4摄氏度下评估7天，以评估冷却损伤指数。结果验证了MyB80基因与番茄植物的耐寒性有关，并且MyB80基因的沉默降低了抗寒能力。

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  • 作者

    Chen Xiuhua; Duan Xuefen; Wang Shuai; Wu Weiyuan; Zhang Xichun;

  • 作者单位

    Beijing Agr Univ Plant Sci &

    Technol Coll Beijing Key Lab New Technol Agr Applicat Beijing 102206 Peoples R China;

    Beijing Agr Univ Plant Sci &

    Technol Coll Beijing Key Lab New Technol Agr Applicat Beijing 102206 Peoples R China;

    Beijing Agr Univ Plant Sci &

    Technol Coll Beijing Key Lab New Technol Agr Applicat Beijing 102206 Peoples R China;

    Beijing Agr Univ Plant Sci &

    Technol Coll Beijing Key Lab New Technol Agr Applicat Beijing 102206 Peoples R China;

    Beijing Agr Univ Plant Sci &

    Technol Coll Beijing Key Lab New Technol Agr Applicat Beijing 102206 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞形态学;
  • 关键词

    Virus-induced gene silencing (VIGS); Tomato; TRV-GFP; MYB80;

    机译：病毒诱导的基因沉默（Vigs）;番茄;TRV-GFP;MYB80;

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