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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil.
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Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil.

机译:Sphaerisporangium siamense sp。 11月。,一种从橡胶树流散土壤隔离的放电工会。

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摘要

A Gram-positive aerobic actinomycete, designated SR14.14(T), isolated from the rhizospheric soil of rubber tree was determined taxonomically using a polyphasic approach. The organism contained meso-diaminopimelic acid and the N-acetyl type of peptidoglycan. The predominant menaquinones were MK-9, MK-9(H) and MK-9(H). Madurose was detected in the whole-cell hydrolysates. Mycolic acids were not presented. Major phospholipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol mannoside. Major cellular fatty acid was iso-C(:) and the G+C content was 71.9 mol %. Phylogenetic analysis based on 16S rRNA gene sequence suggested that the isolate belongs to the genus Sphaerisporangium. The sequence similarity value between the strain SR14.14(T) and its closely related species, Sphaerisporangium album, was 97.8%. DNA-DNA hybridization values between them were well below 70%. Based on genotypic and phenotypic data, strain SR14.14(T) represents a novel species in the genus Sphaerisporangium, for which the name Sphaerisporangium siamense sp. nov. is proposed. The type strain is SR14.14(T) (=BCC 41491(T)=NRRL B-24805(T)=NBRC 107570(T)).
机译:使用多色方法,从橡胶树的relizoSpheric土壤中分离出来的革兰氏阳性的有氧运动,指定的SR14.14(t)。生物体含有甲基二氨基甲酸和N-乙酰基的肽聚糖。主要的母牛是MK-9,MK-9(H)和MK-9(H)。在全细胞水解产物中检测到MADURESE。没有呈现氰酸。主要磷脂是二磷脂酰甘油,磷脂酰乙醇胺和磷脂酰肌醇甘露糖苷。主要细胞脂肪酸是ISO-C(:),G + C含量为71.9mol%。基于16S rRNA基因序列的系统发育分析表明,孤立属属于斯普利桑那州属。菌株SR14.14(T)与其密切相关的物种,Sphaerisporangium专辑的序列相似值为97.8%。它们之间的DNA-DNA杂交值远低于70%。基于基因型和表型数据,菌株SR14.14(T)代表了斯普利兰芒市属的新种类,名称Sphaerisporangium Siamense Sp。 11月。提出。类型应变为SR14.14(t)(= bcc 41491(t)= nrrl b-24805(t)= nbrc 107570(t))。

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