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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Evaluation of solid (disc diffusion)- and liquid (turbidity)-phase antibiogram methods for clinical isolates of diarrheagenic E. coli and correlation with efflux.
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Evaluation of solid (disc diffusion)- and liquid (turbidity)-phase antibiogram methods for clinical isolates of diarrheagenic E. coli and correlation with efflux.

机译：实体（盘扩散） - 和液体（浊度）抗性腹泻的临床分离株的临床分离液和与流出相关性的评价。

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Multiple drug resistance (MDR) in bacteria causes higher mortality and morbidity, complicates treatment and increases health-care outlay. With no new-generation antibiotics in sight, its rapid spread through the environment poses grave danger. Therefore, rapid detection to identify effective antibiotics and to prevent their indiscriminate use is imperative. However, the widely used clinical method for antibiogram, the Kirby-Bauer disc-diffusion method (DDM), requires 2-3 days, has inherent shortcomings of solid-phase assays and is not suitable for high-throughput operations. In our research on MDR associated with childhood diarrhea, we determined the antibiogram of 73 clinical diarrheagenic Escherichia coli strains using both the DDM and the more reliable liquid turbidity method (LTM) performed in 96-microwell plates. The results were further correlated with a dye-exclusion efflux assay using fluorescein diacetate. Although LTM is apparently superior in saving critical time, suitability to high-throughput operations and reliability, we found that the serious shortcomings of DDM could be effectively countered by just doubling the dosage of antibiotics currently used in discs or by using two discs in place of one. With 48 of the 49 MDR strains being positive for efflux and the 12 strains 'susceptible' to all the antibiotics being negative, the efflux assay could be useful as an integral component of the antibiogram test or for additional confirmation. The presence of 65% of MDR strains among diarrheagenic E. coli is a matter of serious concern, although most of them could be treated with either Gentamycin or Amikacin, as is the practice by experience.
机译：细菌中多种耐药性（MDR）导致较高的死亡率和发病率，使治疗变得复杂，并增加了保健支出。没有新一代抗生素在视线中，它通过环境的快速传播构成了严重的危险。因此，快速检测以鉴定有效的抗生素并防止其滥用使用是必要的。然而，广泛使用的抗诊断临床方法，Kirby-Bauer盘扩散方法（DDM）需要2-3天，具有固相测定的固有缺点，不适合高通量操作。在我们对与儿童腹泻相关的MDR的研究中，我们确定了使用DDM和更可靠的液体浊度法（LTM）在96微孔板中进行的73临床腹泻大肠杆菌菌株的抗性。结果与使用荧光素二乙酸酯的染料排除型流出测定进一步相关。虽然LTM在节省关键时间内显然优越，适合高通量的运营和可靠性，但我们发现，只需将目前在光盘中使用的抗生素的剂量或通过使用两个圆盘即可将DDM的严重缺点有效地抵消一。 49个MDR菌株中的48个菌株的阳性，并且对所有抗生素的12个菌株对阴性的菌株“易感”，流出测定可用作抗性测试的一体组分或用于额外的确认。腹泻的患者中有65％的MDR菌株是一个严重关切的问题，尽管大多数人可以用庆大霉素或阿米卡星治疗，也是经验的做法。

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