...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Antibiotics: An International Journal >Identification of a novel bacteriocin-like protein and structural gene from Rhodococcus erythropolis JCM 2895, using suppression-subtractive hybridization
【24h】

Identification of a novel bacteriocin-like protein and structural gene from Rhodococcus erythropolis JCM 2895, using suppression-subtractive hybridization

机译：使用抑制减去杂交的rhodococcus eRerthopolis JCM 2895的鉴定新的细菌样蛋白和结构基因。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel bacteriocin-like protein and its structural gene (rap) were identified from Rhodococcus erythropolis JCM 2895. The rapA and B genes are located on a 5.4-kb circular plasmid, and were obtained using a modified suppression-subtractive hybridization method. The rapA and B genes were heterologously expressed in Rhodococcus sp. or Escherichia coli, and then characterized. The results indicated that RapA is a small, water-soluble, heat-stable antimicrobial protein, and that RapB is an immunity protein against RapA, estimated to be located on the cell membrane. RapA showed antimicrobial activity particularly against R. erythropolis, and the activity persisted even after SDS-PAGE analysis. For the heterologous expressed RapA protein, N-terminal amino acid sequence was also confirmed. This is the first report of a bacteriocin-like substance obtained from the genus Rhodococcus.
机译：从rhodococcus射频JCM 2895鉴定了一种新的细菌素样蛋白及其结构基因（RAP）。RAPA和B基因位于5.4kb圆形质粒上，并使用改性抑制 - 减去杂交方法获得。 Rapa和B基因在rhodococcus sp中异源地表达。 或大肠杆菌，然后表征。 结果表明，RAPA是一种小，水溶性，热稳定的抗微生物蛋白，并且RabB是抗RAPA的免疫蛋白，估计位于细胞膜上。 Rapa表现出抗菌活性，特别是针对r. erythopolis，即使在SDS-PAGE分析后，活动也持续存在。 对于异源表达的RAPA蛋白，还证实了N-末端氨基酸序列。 这是从杜鹃花中获得的噬菌体样物质的第一份报告。

著录项

  • 来源
  • 作者

    Kitagawa Wataru; Mitsuhashi Shinya; Hata Miyako; Tamura Tomohiro;

  • 作者单位

    Natl Inst Adv Ind Sci &

    Technol Toyohira ku 2-17-2-1 Tsukisamu Higashi Sapporo Hokkaido 0628517;

    Hokkaido Univ Grad Sch Agr Kita Ku Kita 9 Nishi 9 Sapporo Hokkaido 0608589 Japan;

    Natl Inst Adv Ind Sci &

    Technol Toyohira ku 2-17-2-1 Tsukisamu Higashi Sapporo Hokkaido 0628517;

    Natl Inst Adv Ind Sci &

    Technol Toyohira ku 2-17-2-1 Tsukisamu Higashi Sapporo Hokkaido 0628517;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号