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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Differential expression analysis of the SRB1 gene in fluconazole-resistant and susceptible strains of Candida albicans
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Differential expression analysis of the SRB1 gene in fluconazole-resistant and susceptible strains of Candida albicans

机译：甘蔗唑抗性易受敏感菌株抗脂菌和敏感菌株的SRB1基因的差异表达分析

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摘要

To study differences in SRB1 gene expression between Candida albicans fluconazole-resistant strains and fluconazole-sensitive strains, and to identify new antifungal drug treatment targets. We studied 30 fluconazole-resistant and 47 fluconazole-sensitive C. albicans strains. The strains were routinely cultured, and total RNA was extracted, reverse transcribed into cDNA and analyzed with real-time PCR amplification with 18S rRNA used as an internal reference gene. The expression levels of the two groups were analyzed in Light Cycler system software version 3.0, and independent Student's t test was performed in SPSS 19.0 statistical software. P < 0.05 was considered to indicate a statistical difference. DNAMAN multiple sequence alignment analysis was used to randomly analyze SBR1 related sequences of four resistant strains and four sensitive strains. An evolutionary tree was constructed with the maximum likelihood method in Mega6.0 software. The mean SRB1 gene expression in the drug-resistant group was 0.75138 x 10(-3), and that in the sensitive group was 1.6664 x 10(-3). Independent Student's t test indicated a statistically significant difference (T = -3.972, P = 0.000, P < 0.05). DNAMAN multiple sequence alignment analysis showed that the sequence identity of the CDS in the eight strains was 75.17%, and that of sequences 1000 bp upstream of the CDS was 96.35%. Cluster analysis showed that the CDS and sequences 1000 bp upstream of the CDS showed no significant differences between groups. At the mRNA level, the SRB1 gene expression in fluconazole-resistant C. albicans was lower than that in fluconazole-sensitive strains, thus suggesting that the gene may be associated with drug resistance and that the regulatory mechanism leading to this difference is complex.
机译：研究念珠菌抗氟康唑抗性菌株和氟康唑敏感菌株的SRB1基因表达的差异，并鉴定新的抗真菌药物治疗靶标。我们研究了30个氟康唑抗性和47个氟康唑敏感的C. albicans菌株。常规培养菌株，并提取总RNA，转录成cDNA并用实时PCR扩增分析，用18S rRNA作为内部参考基因。两组的表达水平分析在轻循环系统软件3.0版本3.0中，并在SPSS 19.0统计软件中进行独立的学生的T测试。 P <0.05被认为表示统计学差异。 DNAMAN多序列对准分析用于随机分析四种抗性菌株和四种敏感菌株的SBR1相关序列。进化树是在Mega6.0软件中的最大似然方法构建的。耐药基团中的平均SRB1基因表达为0.75138×10（-3），并且在敏感基团中为1.6664×10（-3）。独立学生的T检验表明统计学上有显着差异（T = -3.972，P = 0.000，P <0.05）。 DNAMAN多序列对准分析表明，八种菌株中CD的序列同一性为75.17％，CD上游序列1000bp为96.35％。聚类分析表明，CD上游的CD和序列1000bp在群体之间没有显着差异。在mRNA水平下，氟康唑抗性C.敏感剂中的SRB1基因表达低于氟康唑敏感菌株的抗性，因此表明该基因可能与耐药有关，并且导致这种差异的调节机制是复杂的。

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    Ji Lingyun; Zhou Aiping; Yu Xiaoyu; Dong Zhenghua; Zhao Huaping; Xue Hui; Wu Wenjuan;

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    Tongji Univ Sch Med Shanghai EastHosp Dept Lab Med Shanghai 200123 Peoples R China;

    Tongji Univ Sch Med Shanghai EastHosp Dept Lab Med Shanghai 200123 Peoples R China;

    Tongji Univ Sch Med Shanghai EastHosp Dept Lab Med Shanghai 200123 Peoples R China;

    Tongji Univ Sch Med Shanghai EastHosp Dept Lab Med Shanghai 200123 Peoples R China;

    Tongji Univ Sch Med Shanghai EastHosp Dept Lab Med Shanghai 200123 Peoples R China;

    Tongji Univ Sch Med Shanghai EastHosp Dept Lab Med Shanghai 200123 Peoples R China;

    Tongji Univ Sch Med Shanghai EastHosp Dept Lab Med Shanghai 200123 Peoples R China;

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