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首页> 外文期刊>The ISME journal emultidisciplinary journal of microbial ecology >Bacterial vitamin B-12 production enhances nematode predatory behavior
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Bacterial vitamin B-12 production enhances nematode predatory behavior

机译：细菌维生素B-12生产增强了线虫掠夺性行为

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Although the microbiota is known to affect host development, metabolism, and immunity, its impact on host behavior is only beginning to be understood. In order to better characterize behavior modulation by host-associated microorganisms, we investigated how bacteria modulate complex behaviors in the nematode model organism Pristionchus pacificus. This nematode is a predator that feeds on the larvae of other nematodes, including Caenorhabditis elegans. By growing P. pacificus on different bacteria and testing their ability to kill C. elegans, we reveal large differences in killing efficiencies, with a Novosphingobium species showing the strongest enhancement. This enhanced killing was not accompanied by an increase in feeding, which is a phenomenon known as surplus killing, whereby predators kill more prey than necessary for sustenance. Our RNA-seq data demonstrate widespread metabolic rewiring upon exposure to Novosphingobium, which facilitated screening of bacterial mutants with altered transcriptional responses. We identified bacterial production of vitamin B-12 as an important cause of such enhanced predatory behavior. Although vitamin B-12 is an essential cofactor for detoxification and metabolite biosynthesis, shown previously to accelerate development in C. elegans, supplementation with this enzyme cofactor amplified surplus killing in P. pacificus, whereas mutants in vitamin B-12-dependent pathways reduced surplus killing. By demonstrating that production of vitamin B-12 by host-associated microbiota can affect complex host behaviors, we reveal new connections between animal diet, microbiota, and nervous system.
机译：虽然已知微生物会影响宿主开发，新陈代谢和免疫力，但它对宿主行为的影响仅开始被理解。为了更好地表征宿主相关的微生物的行为调节，我们研究了细菌如何调节线虫模型生物胰岛素Pacificus的复杂行为。该线虫是一种捕食者，其在其他线虫的幼虫，包括Caenorhabditis elegans。通过在不同的细菌上生长P. Pacificus并测试他们杀死C.杆状花虫的能力，我们揭示了杀伤效率的巨大差异，具有呈现最强的增强性的缺钙毒性物种。这种增强的杀戮并不伴随着饲养的增加，这是一种称为剩余杀戮的现象，从而捕食者杀死比寄托所需的更多猎物。我们的RNA-SEQ数据在暴露于Novosphingo obium时展示了广泛的代谢再次促进，这促进了具有改变的转录反应的细菌突变体的筛选。我们确定了维生素B-12的细菌生产是这种增强的掠夺性行为的重要原因。虽然维生素B-12是用于解毒和代谢物生物合成的必需辅助因子，但先前为了加速C.杆骨的发育，补充于P. Pacificus的这种酶Cofactor扩增的剩余杀死，而维生素B-12依赖性途径的突变体减少过剩杀戮。通过展示通过宿主相关的微生物群生产的维生素B-12可以影响复杂的宿主行为，我们揭示了动物饮食，微生物群和神经系统之间的新联系。

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    Akduman Nermin; Lightfoot James W.; Roeseler Waltraud; Witte Hanh; Lo Wen-Sui; Roedelsperger Christian; Sommer Ralf J.;

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    Max Planck Inst Dev Biol Dept Evolutionary Biol Max Planck Ring 9 D-72076 Tubingen Germany;

    Max Planck Inst Dev Biol Dept Evolutionary Biol Max Planck Ring 9 D-72076 Tubingen Germany;

    Max Planck Inst Dev Biol Dept Evolutionary Biol Max Planck Ring 9 D-72076 Tubingen Germany;

    Max Planck Inst Dev Biol Dept Evolutionary Biol Max Planck Ring 9 D-72076 Tubingen Germany;

    Max Planck Inst Dev Biol Dept Evolutionary Biol Max Planck Ring 9 D-72076 Tubingen Germany;

    Max Planck Inst Dev Biol Dept Evolutionary Biol Max Planck Ring 9 D-72076 Tubingen Germany;

    Max Planck Inst Dev Biol Dept Evolutionary Biol Max Planck Ring 9 D-72076 Tubingen Germany;

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  • 正文语种 eng
  • 中图分类 微生物学;
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