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首页> 外文期刊>The Journal of Nutritional Biochemistry >Dietary polyunsaturated fatty acids modulate adipose secretome and is associated with changes in mammary epithelial stem cell self-renewal
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Dietary polyunsaturated fatty acids modulate adipose secretome and is associated with changes in mammary epithelial stem cell self-renewal

机译：膳食多不饱和脂肪酸调节脂肪沉淀物，与乳腺上皮干细胞自更新的变化有关

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Chronic low-grade adipose inflammation, characterized by aberrant adipokine production and pro-inflammatory macrophage activation/polarization is associated with increased risk of breast cancer. Adipocyte fatty acid composition is influenced by dietary availability and may regulate adipokine secretion and adipose inflammation. After feeding F344 rats for 20 weeks with a Western diet or a fish oil-supplemented diet, we cultured primary rat adipose tissue in a three-dimensional explant culture and collected the conditioned medium. The rat adipose tissue secretome was assayed using the Proteome Profiler Cytokine XL Array, and adipose tissue macrophage polarization (M1 /M2 ratio) was assessed using the iNOS/ARG1 ratio. We then assessed the adipokine's effects upon stem cell self-renewal using primary human mammospheres from normal breast mammoplasty tissue. Adipose from rats fed the fish oil diet had an omega-3:omega-6 fatty acid ratio of 0.28 compared to 0.04 in Western diet rats. The adipokine profile from the fish oil-fed rats was shifted toward adipokines associated with reduced inflammation compared to the rats fed the Western diet. The M1 /M2 macrophage ratio decreased by 50% in adipose of fish oil-fed rats compared to that from rats fed the Western diet. Conditioned media from rats fed the high omega-6 Western diet increased stem cell self-renewal by 62%+/- 9% ((X) over bar%+/- SD) above baseline compared to only an 11%+/- 11% increase with the fish oil rat adipose. Modulating the adipokine secretome with dietary interventions therefore may alter stromal-epithelial signaling that plays a role in controlling mammary stem cell self-renewal. (C) 2019 Elsevier Inc. All rights reserved.
机译：慢性低级脂肪炎症，其特征在于异哌啶生产和促炎巨噬细胞激活/偏振，与乳腺癌的风险增加有关。脂肪细胞脂肪酸组合物受膳食可用性的影响，可调节己脒分泌和脂肪炎症。用西方饮食或鱼油补充饮食喂养20周后的F344大鼠，我们培养了原发性大鼠脂肪组织在三维外植体培养物中并收集条件培养基。使用蛋白质组分布细胞因子X1阵列测定大鼠脂肪组织克制，并且使用INOS / ARG1的比率评估脂肪组织巨噬细胞极化（M1 / M2比率）。然后，我们使用来自正常乳腺乳腺术组织的原发性人乳腺间体评估adipokine对干细胞自我更新的影响。来自喂养鱼油饮食的大鼠的脂肪有ω-3：ω-6脂肪酸比为0.28，与西方饮食大鼠的0.04相比。与喂养西方饮食的大鼠相比，来自鱼油喂养大鼠的己鸟类曲线朝向与炎症的降低相关的脂肪因子。与喂养西方饮食的大鼠相比，M1 / M2巨噬细胞比率下降了50％的鱼油喂养大鼠脂肪。来自大鼠的调节培养基，喂养高ω-6西方饮食的干细胞自我更新，通过高于基线的62％+ / - 9％（（x）上方的酒吧％+ / - sd），而仅为11％+ / - 11用鱼油大鼠脂肪增加％。因此，调节饮食干预的己酮沉淀可以改变在控制乳腺干细胞自我更新中起作用的基质上皮信号。 （c）2019 Elsevier Inc.保留所有权利。

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