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首页> 外文期刊>The Journal of Nutritional Biochemistry >Dietary supplementation with strawberry induces marked changes in the composition and functional potential of the gut microbiome in diabetic mice
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Dietary supplementation with strawberry induces marked changes in the composition and functional potential of the gut microbiome in diabetic mice

机译：用草莓膳食补充剂在糖尿病小鼠中诱导肠道微生物组的组成和功能潜力的显着变化

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Gut microbiota contributes to the biological activities of berry anthocyanins by transforming them into bioactive metabolites, and anthocyanins support the growth of specific bacteria, indicating a two-way relationship between anthocyanins and microbiota. In the present study, we tested the hypothesis that strawberry supplementation alters gut microbial ecology in diabetic db/db mice. Control (db/+) and diabetic (db/db) mice (7 weeks old) consumed standard diet or diet supplemented with 235% freeze-dried strawberry (db/db + SB) for 10 weeks. Colon contents were used to isolate bacterial DNA. V4 variable region of 16S rRNA gene was amplified. Data analyses were performed using standardized pipelines (QIIME 1.9 and R packages). Differences in predictive metagenomics function were identified by PICRUSt. Principal coordinate analyses confirmed that the microbial composition was significantly influenced by both host genotype and strawberry consumption. Further, alpha-diversity indices and beta-diversity were different at the phylum and genus levels, and genus and operational taxonomical units levels, respectively (P.05). At the phylum level, strawberry supplementation decreased the abundance of Verrucomicrobia in db/db + SB vs. db/db mice (P.05). At the genus level, db/db mice exhibited a decrease in the abundance of Bifidobacterium, and strawberry supplementation increased Bifidobacterium in db/db + SB vs. db/db mice (P.05). PICRUSt revealed significant differences in 45 predicted metabolic functions among the 3 groups. Our study provides evidence for marked changes in the composition and functional potential of the gut microbiome with strawberry supplementation in diabetic mice. Importantly, strawberry supplementation increased the abundance of beneficial bacteria Bifidobacterium which play a pivotal role in the metabolism of anthocyanins. (C) 2019 Elsevier Inc. All rights reserved.
机译：通过将它们转化为生物活性代谢物，肠道微生物菌促进了莓果花青素的生物学活性，并且花青素支持特定细菌的生长，表明花青素和微生物群之间的双向关系。在本研究中，我们测试了草莓补充在糖尿病DB / DB小鼠中改变了肠道微生物生态的假设。对照（DB / +）和糖尿病（DB / DB）小鼠（7周龄）消耗的标准饮食或补充有235％冻干草莓（DB / DB + SB）的饮食10周。结肠内容物用于分离细菌DNA。扩增了16S rRNA基因的V4可变区。使用标准化管道（Qiime 1.9和R包）进行数据分析。通过屠杀鉴定了预测性偏心神经功能的差异。主坐标分析证实微生物组合物受到宿主基因型和草莓消耗的显着影响。此外，α-多样性指数和β-多样性分别在文学和属水平和属的属性中不同（P <.05）。在门水平下，草莓补充在DB / DB + Sb与DB / DB小鼠中的verrucoMisrobia的丰度降低（P <.05）。在Genus水平，DB / DB小鼠表现出丰富的双歧杆菌的减少，草莓补充在DB / DB + Sb与DB / dB小鼠中增加双歧杆菌（P <.05）。 Picrust在3组中揭示了45个预测代谢功能的显着差异。我们的研究提供了在糖尿病小鼠中与草莓补充的肠道微生物组的组成和功能潜力的显着变化的证据。重要的是，草莓补充剂增加了有益细菌双歧杆菌的丰富，这在花青素的代谢中起着枢转作用。 （c）2019 Elsevier Inc.保留所有权利。

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