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首页> 外文期刊>The journals of gerontology.Series A. Biological sciences and medical sciences >Effects of Calorie Restriction and Fiber Type on Glucose Uptake and Abundance of Electron Transport Chain and Oxidative Phosphorylation Proteins in Single Fibers from Old Rats
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Effects of Calorie Restriction and Fiber Type on Glucose Uptake and Abundance of Electron Transport Chain and Oxidative Phosphorylation Proteins in Single Fibers from Old Rats

机译：旧大鼠单纤维中葡萄糖限制和纤维型对葡萄糖摄取及氧化磷酸化蛋白的影响

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Calorie restriction (CR; reducing calorie intake by similar to 40% below ad libitum) can increase glucose uptake by insulin-stimulated muscle. Because skeletal muscle is comprised of multiple, heterogeneous fiber types, our primary aim was to determine the effects of CR (initiated at 14 weeks old) and fiber type on insulin-stimulated glucose uptake by single fibers of diverse fiber types in 23-26-month-old rats. Isolated epitrochlearis muscles from AL and CR rats were incubated with [H-3]-2-deoxyglucose +/- insulin. Glucose uptake and fiber type were determined for single fibers dissected from the muscles. We also determined CR-effects on abundance of several key metabolic proteins in single fibers. CR resulted in: (a) significantly (p < .05 to .001) greater glucose uptake by insulin-stimulated type I, IIA, IIB, IIBX, and IIX fibers; (b) significantly (p < .05 to .001) reduced abundance of several mitochondrial electron transport chain (ETC) and oxidative phosphorylation (OxPhos) proteins in type I, IIA, and IIBX but not IIB and IIX fibers; and (c) unaltered hexokinase II abundance in each fiber type. These results demonstrate that CR can enhance glucose uptake in each fiber type of rat skeletal muscle in the absence of upregulation of the abundance of hexokinase II or key mitochondrial ETC and OxPhos proteins.
机译：卡路里限制（CR;通过低于AD Libitum的40％降低卡路里摄入量）可以通过胰岛素刺激的肌肉增加葡萄糖摄取。由于骨骼肌由多种异质纤维类型组成，因此我们的主要目的是确定Cr（在14周龄衰老）和纤维型胰岛素刺激葡萄糖摄取的效果，在23-26中的单纤维通过单纤维产生胰岛素刺激的葡萄糖摄取。月大的老鼠。来自Al和Cr大鼠的孤立的ePitrochlearis肌肉与[H-3] -2-脱氧葡萄糖+/-胰岛素孵育。测定葡萄糖摄取和纤维型，用于从肌肉中解剖的单纤维。我们还确定了对单纤维中几种关键代谢蛋白的丰度的Cr-use。 CR导致：（a）显着（p <.05至0.001）胰岛素刺激的I，IIa，IIB，IIBX和IIX纤维更大的葡萄糖摄取; （b）显着（p <.05至0.001）在I，IIa和IIBX I类，IIBX中的几种线粒体电子传输链（ETC）和氧化磷酸化（毒物）蛋白的丰度降低，但不是IIB和IIX纤维; （c）在每种纤维类型中未改变六酮酶II丰度。这些结果表明，在没有上调六酮酶II或关键线粒体等和毒物蛋白质的情况下，Cr可以增强每种纤维类型的大鼠骨骼肌中的葡萄糖摄取。

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