• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Rapid calibration of near-infrared spectroscopic measurements of mammaliancell cultivations
【24h】

Rapid calibration of near-infrared spectroscopic measurements of mammaliancell cultivations

机译：哺乳动物细胞培养物的近红外光谱测量的快速校准

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Near-infrared (NIR) spectroscopy is a flexible method that can be employed to noninvasively monitor the concentrations of multiple nutrients and wastes in mammalian cell bioreactors. Development of suitable calibrations can be a labor- and time-intensive process that must be repeated when process conditions are altered significantly. To address this difficulty, we have produced a new approach for generating NIR spectroscopic calibrations that requires significantly less time compared with standard calibration schemes. This method reduces development time from the present level of several weeks to several hours. A small number of experimentally collected spectra serve as inputs to a computational procedure that yields a large number of simulated spectra, each containing both analyte-specific and analyte-independent information. Such simulated spectra may be employed as a calibration set for quantifying analytes in experimentally collected spectra. Spectroscopic measurements of the concentrations of five components (ammonia, glucose, glutamate, glutamine, and lactate) can be accomplished with levels of error similar to those obtained with full experimental calibrations. A key to this process is the utilization of random numbers, which randomizes the influence of natural variations, present in each experimentally collected spectrum, on the resultant composite spectrum. This approach may increase the feasibility of employing NIR spectroscopy to monitor bioreactors and other biological processes subjected to varying operating conditions.
机译：近红外（NIR）光谱法是一种灵活的方法，可用于无创地监测哺乳动物细胞生物反应器中多种营养物质和废物的浓度。合适的校准的开发可能是一项劳动和时间密集的过程，当过程条件发生重大变化时必须重复进行。为了解决这一难题，我们开发了一种用于生成NIR光谱校准的新方法，与标准校准方案相比，该方法所需的时间要少得多。这种方法将开发时间从目前的几周减少到几小时。少量实验收集的光谱用作计算过程的输入，该计算过程会产生大量模拟光谱，每个光谱均包含特定于分析物的信息和与分析物无关的信息。这样的模拟光谱可以用作用于量化实验收集的光谱中的分析物的校准集。可以对五种成分（氨，葡萄糖，谷氨酸盐，谷氨酰胺和乳酸盐）的浓度进行光谱测量，其误差水平与通过完全实验校准获得的误差水平相似。此过程的关键是利用随机数，该随机数将每个实验收集的光谱中存在的自然变化对所得合成光谱的影响随机化。这种方法可能会增加使用NIR光谱法监测生物反应器和其他生物过程在不同操作条件下的可行性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号