• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Single, combined, or sequential action of pressure and temperature onlipoxygenase in green beans (Phaseolus vulgaris L): A kinetic inactivationstudy
【24h】

Single, combined, or sequential action of pressure and temperature onlipoxygenase in green beans (Phaseolus vulgaris L): A kinetic inactivationstudy

机译：绿豆（菜豆）中压力和温度脂氧合酶的单一，组合或顺序作用：动力学失活研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objective of this investigation was to study kinetically the effect of pressure and temperature either as a single, as a combined, or as a sequential action on Lipoxygenase (LOX) inactivation in crude green beans extract. The LOX isozymes in green beans extract had a different heat sensitivity but a similar pressure stability: two fractions following apparent first-order reactions, i.e., a heat-labile fraction and a heat-stable fraction, were observed in studies on its thermostability, whereas only one fraction following a first-order reaction was noticed in studies on its pressure stability. At ambient pressure, irreversible LOX inactivation was studied in a temperature range from 55 to 70 degrees C. At room temperature, pressures around 500 MPa were required in order to inactivate LOX in green beans extract. The effect of a pressure or a thermal pretreatment on LOX thermo- or barostability, respectively, was also investigated but no significant differences in inactivation kinetics due to the pretreatment were observed.
机译：这项研究的目的是从动力学角度研究压力和温度对生豆提取物中脂氧合酶（LOX）失活的影响，既可以是单一作用，也可以是组合作用，也可以是顺序作用。绿豆提​​取物中的LOX同工酶具有不同的热敏感性，但具有相似的压力稳定性：在研究其热稳定性时，观察到明显的一级反应后的两个馏分，即热不稳定馏分和热稳定馏分，而在其压力稳定性的研究中，仅注意到一级反应后的一部分。在环境压力下，研究了55至70摄氏度的温度下不可逆LOX失活。在室温下，需要约500 MPa的压力才能使青豆提取物中的LOX失活。还研究了压力或热预处理分别对LOX热或耐压性的影响，但未观察到由于预处理导致的失活动力学有显着差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号